Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Microbiol Immunol. 2012 May;56(5):332-7. doi: 10.1111/j.1348-0421.2012.00438.x.
A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine after the initial inoculation. A subsequent vaccination-challenge test showed an increased PD(50) value. Thus, coadministration of the recombinant plasmid with the oil-adjuvant vaccine helped illicit an immune response earlier than that elicited by giving the vaccine alone. Our results showed that pUC18-CpG can be a potent immunoadjuvant for the traditional FMD killed vaccine and can greatly enhance the traditional vaccine's efficacy when given in combination with it.
一种富含 CpG 的重组质粒（pUC18-CpG）作为口蹄疫灭活疫苗的佐剂，在猪模型中进行了免疫和接种挑战测试。我们的初步结果表明，该重组质粒可以增强传统油佐剂疫苗初次接种后的体液免疫反应。随后的接种挑战测试显示 PD（50）值增加。因此，与油佐剂疫苗联合使用重组质粒有助于比单独给予疫苗更早引发免疫反应。我们的结果表明，pUC18-CpG 可以作为传统口蹄疫灭活疫苗的有效免疫佐剂，与传统疫苗联合使用时可以大大提高其疗效。
