University of Science and Technology, Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
Bioresour Technol. 2012 Apr;110:552-9. doi: 10.1016/j.biortech.2012.01.079. Epub 2012 Jan 26.
The freshwater microalga, Hydrodictyon reticulum, that contained 47.5% reducing sugars including 35% glucose was used as substrate for the production of l-lactic acid (LA) by LA-producing bacteria. Lactobacillus paracasei LA104 was selected for fermentation in a 5-l fermentor since it was able to grow at pH 3, 60g LA/l, 200g glucose/l, 125g NaCl/l, and 45°C and produced over 97.3% optically pure l-lactic acid with glucose as a substrate. Simultaneous saccharification and cofermentation from H. reticulum to l-LA using LA104 was investigated in a jar fermentor. The yield reached 46g/100g H. reticulum dry material, with a final concentration of 37.11g/l and a productivity of 1.03g/l/h. This is the first report of the production of l-LA from a microalga, and H. reticulum could be a potential feedstock for large-scale production of l-LA by LA104.
以含有 47.5%还原糖（其中 35%为葡萄糖）的淡水微藻水网藻为原料，利用产乳酸细菌生产 L-乳酸（LA）。由于能够在 pH 3、60g LA/L、200g 葡萄糖/L、125g NaCl/L 和 45°C 下生长，并且以葡萄糖为底物可生产超过 97.3%的光学纯 L-乳酸，因此选择副干酪乳杆菌 LA104 进行 5-L 发酵罐发酵。在摇瓶发酵罐中，使用 LA104 对水网藻进行同步糖化和共发酵生产 L- LA。产率达到 46g/100g 水网藻干物质，最终浓度为 37.11g/L，生产强度为 1.03g/L/h。这是首次报道从微藻生产 L- LA，水网藻可以作为 LA104 大规模生产 L- LA 的潜在原料。
