apcE 基因缺失的集胞藻 PCC 6803 中生物质产量和糖原积累增加。
Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803.
机构信息
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Hyogo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku 102-0075, Tokyo, Japan.
出版信息
AMB Express. 2014 Mar 15;4:17. doi: 10.1186/s13568-014-0017-z. eCollection 2014.
The effect of phycobilisome antenna-truncation in the cyanobacterium Synechocystis sp. PCC 6803 on biomass production and glycogen accumulation have not yet been fully clarified. To investigate these effects here, the apcE gene, which encodes the anchor protein linking the phycobilisome to the thylakoid membrane, was deleted in a glucose tolerant strain of Synechocystis sp. PCC 6803. Biomass production of the apcE-deleted strain under photoautotrophic and atmospheric air conditions was 1.6 times higher than that of strain PCC 6803 (1.32 ± 0.01 versus 0.84 ± 0.07 g cell-dry weight L(-1), respectively) after 15 days of cultivation. In addition, the glycogen content of the apcE-deleted strain (24.2 ± 0.7%) was also higher than that of strain PCC 6803 (11.1 ± 0.3%). Together, these results demonstrate that antenna truncation by deleting the apcE gene was effective for increasing biomass production and glycogen accumulation under photoautotrophic and atmospheric air conditions in Synechocystis sp. PCC 6803.
藻胆体天线截断对集胞藻 PCC 6803 生物量生产和糖原积累的影响尚未完全阐明。为了研究这些影响，在集胞藻 PCC 6803 的一株耐葡萄糖菌株中敲除了编码将藻胆体与类囊体膜连接的锚蛋白的 apcE 基因。在 15 天的培养后，apcE 缺失菌株在光自养和大气条件下的生物量分别比 PCC 6803 菌株（分别为 1.32 ± 0.01 和 0.84 ± 0.07 g 细胞干重 L-1）高 1.6 倍。此外，apcE 缺失菌株的糖原含量（24.2 ± 0.7%）也高于 PCC 6803 菌株（11.1 ± 0.3%）。这些结果表明，在集胞藻 PCC 6803 中，通过敲除 apcE 基因进行天线截断，在光自养和大气条件下有效提高了生物量生产和糖原积累。
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