Department of Applied Nutrition and Food Technology, Faculty of Applied Science and Technology, Islamic University, Kushtia, Bangladesh.
Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba 305-8575, Japan.
J Gen Virol. 2012 Jun;93(Pt 6):1328-1338. doi: 10.1099/vir.0.036665-0. Epub 2012 Feb 15.
B23/nucleophosmin has been identified in vitro as a stimulatory factor for replication of adenovirus DNA complexed with viral basic core proteins. In the present study, the in vivo function of B23 in the adenovirus life cycle was studied. It was found that both the expression of a decoy mutant derived from adenovirus core protein V that tightly associates with B23 and small interfering RNA-mediated depletion of B23 impeded the production of progeny virions. However, B23 depletion did not significantly affect the replication and transcription of the virus genome. Chromatin immunoprecipitation analyses revealed that B23 depletion significantly increased the association of viral DNA with viral core proteins and cellular histones. These results suggest that B23 is involved in the regulation of association and/or dissociation of core proteins and cellular histones with the virus genome. In addition, these results suggest that proper viral chromatin assembly, regulated in part by B23, is crucial for the maturation of infectious virus particles.
B23/nucleophosmin 在体外被鉴定为一种能够刺激腺病毒 DNA 复制的因子，该 DNA 与病毒基本核心蛋白形成复合物。在本研究中，研究了 B23 在腺病毒生命周期中的体内功能。结果发现，与 B23 紧密结合的腺病毒核心蛋白 V 的诱饵突变体的表达以及 B23 的小干扰 RNA 介导的耗竭均会阻碍子代病毒颗粒的产生。然而，B23 的耗竭并不会显著影响病毒基因组的复制和转录。染色质免疫沉淀分析表明，B23 的耗竭会显著增加病毒 DNA 与病毒核心蛋白和细胞组蛋白的结合。这些结果表明，B23 参与调节核心蛋白和细胞组蛋白与病毒基因组的结合和/或解离。此外，这些结果表明，适当的病毒染色质组装（部分受 B23 调节）对于成熟的感染性病毒颗粒至关重要。
