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[来自假单胞菌噬菌体群体代表的DNA的物理化学性质]

[Physico-chemical properties of DNA from representatives of a population of Pseudomonas bacteriophages].

作者信息

Romashko A M, Bylinskiĭ A F, Ialoshevich A M, Vlasov A P

出版信息

Mikrobiologiia. 1990 Jul-Aug;59(4):616-23.

PMID:2263225
Abstract

Twenty-four bacteriophages of Pseudomonas similar in terms of their morphology and structure were studied. The genomes of 23 bacteriophages from a population widespread in Belorussia and of one bacteriophage from the Krasnodar Region were shown to be double-helical DNAs with the content of GC base pairs from 46.8 to 66.7%. Four endonucleases (Eco R1, Hind III, Bam H1, and Eco RV) were used in the restriction analysis. Phage 8 which differed from the other phages in the area of its distribution was found to represent an individual group. The other phages were subdivided into eight related groups and had an identical restriction map within each group. The mean molecular masses of phage DNAs calculated by the method of summing the molecular masses of restricts ranged from 27.8 to 31.0 MDa.

摘要

对24种形态和结构相似的假单胞菌噬菌体进行了研究。结果表明,来自白俄罗斯广泛分布群体的23种噬菌体和来自克拉斯诺达尔地区的1种噬菌体的基因组为双螺旋DNA,GC碱基对含量为46.8%至66.7%。在限制性分析中使用了四种核酸内切酶(Eco R1、Hind III、Bam H1和Eco RV)。发现噬菌体8在其分布区域与其他噬菌体不同,代表一个单独的组。其他噬菌体被细分为八个相关组,每组内具有相同的限制性图谱。通过对限制性片段分子质量求和的方法计算出的噬菌体DNA平均分子质量范围为27.8至31.0 MDa。

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