Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.
J Am Chem Soc. 2012 Mar 7;134(9):3965-7. doi: 10.1021/ja2108905. Epub 2012 Feb 24.
Understanding the interactions of nanomaterials with the immune system is essential for the engineering of new macromolecular systems for in vivo applications. Systematic study of immune activation is challenging due to the complex structure of most macromolecular probes. We present here the use of engineered gold nanoparticles to determine the sole effect of hydrophobicity on the immune response of splenocytes. The gene expression profile of a range of cytokines (immunological reporters) was analyzed against the calculated log P of the nanoparticle headgroups, with an essentially linear increase in immune activity with the increase in hydrophobicity observed in vitro. Consistent behavior was observed with in vivo mouse models, demonstrating the importance of hydrophobicity in immune system activation.
了解纳米材料与免疫系统的相互作用对于体内应用的新型大分子系统的工程设计至关重要。由于大多数大分子探针的复杂结构，系统地研究免疫激活具有挑战性。我们在这里介绍了使用工程金纳米粒子来确定疏水性对脾细胞免疫反应的单一影响。针对纳米粒子头基的计算 log P，分析了一系列细胞因子（免疫报告基因）的基因表达谱，观察到体外免疫活性随疏水性增加呈基本线性增加。在体内小鼠模型中观察到一致的行为，证明了疏水性在免疫系统激活中的重要性。
