通过缺乏脂磷壁酸的嗜酸乳杆菌调节肠道免疫反应。
Modulating intestinal immune responses by lipoteichoic acid-deficient Lactobacillus acidophilus.
机构信息
Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
出版信息
Immunotherapy. 2012 Feb;4(2):151-61. doi: 10.2217/imt.11.163.
Abstract
AIM
To investigate the mechanism(s) by which the intestinal commensal microbe Lactobacillus acidophilus can affect host immunity, we studied the role of a component of the cell wall, lipoteichoic acid, in colitis.
MATERIALS & METHODS: Colitis was induced by the intraperitoneal injection of pathogenic CD4(+)CD25(-)CD45RB(hi) T cells into Rag1(-/-) mice. The parental strain, NCK56, or the lipoteichoic acid-deficient strain, NCK2025, was then administered orally. Fluorescent microscopy was employed to examine resulting cell populations and their cytokine production in the colon.
RESULTS
NCK2025 enhanced IL-10 production by dendritic cells and macrophages. Increased numbers of regulatory dendritic cells coincided with the induction of activated FoxP3(+) Tregs.
CONCLUSION
These results suggest that the oral administration of the genetically modified strain NCK2025 may be an effective immunotherapeutic approach that reprograms the immune response in colonic inflammatory conditions.
摘要
目的
为了研究肠道共生微生物嗜酸乳杆菌影响宿主免疫的机制，我们研究了细胞壁成分脂磷壁酸在结肠炎中的作用。
材料与方法
通过向 Rag1(-/-) 小鼠腹腔内注射致病性 CD4(+)CD25(-)CD45RB(hi)T 细胞来诱导结肠炎。然后，通过口服给予亲本菌株 NCK56 或脂磷壁酸缺陷菌株 NCK2025。采用荧光显微镜检查结肠中产生的细胞群及其细胞因子的产生。
结果
NCK2025 增强了树突状细胞和巨噬细胞中 IL-10 的产生。调节性树突状细胞数量的增加与激活的 FoxP3(+)Treg 的诱导同时发生。
结论
这些结果表明，口服给予基因修饰的 NCK2025 菌株可能是一种有效的免疫治疗方法，可以重塑结肠炎症条件下的免疫反应。
相似文献
1
Modulating intestinal immune responses by lipoteichoic acid-deficient Lactobacillus acidophilus.通过缺乏脂磷壁酸的嗜酸乳杆菌调节肠道免疫反应。
Immunotherapy. 2012 Feb;4(2):151-61. doi: 10.2217/imt.11.163.
2
Lipoteichoic acid-deficient Lactobacillus acidophilus regulates downstream signals.缺失脂磷壁酸的嗜酸乳杆菌调节下游信号。
Immunotherapy. 2011 Mar;3(3):337-47. doi: 10.2217/imt.10.119.
3
Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid.脂磷壁酸缺陷型嗜酸乳杆菌对诱导性结肠炎症的调控作用。
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4623-30. doi: 10.1073/pnas.1005066107. Epub 2011 Jan 31.
4
The impact of Lactobacillus plantarum WCFS1 teichoic acid D-alanylation on the generation of effector and regulatory T-cells in healthy mice.植物乳杆菌 WCFS1 磷壁酸 D-丙氨酸化对健康小鼠效应器和调节性 T 细胞产生的影响。
PLoS One. 2013 Apr 30;8(4):e63099. doi: 10.1371/journal.pone.0063099. Print 2013.
5
Abating colon cancer polyposis by Lactobacillus acidophilus deficient in lipoteichoic acid.通过缺失脂磷壁酸的嗜酸乳杆菌来减少结肠息肉。
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10462-7. doi: 10.1073/pnas.1207230109. Epub 2012 Jun 11.
6
Characterization of Foxp3+CD4+CD25+ and IL-10-secreting CD4+CD25+ T cells during cure of colitis.结肠炎治愈过程中Foxp3 + CD4 + CD25 +和分泌IL - 10的CD4 + CD25 + T细胞的特征
J Immunol. 2006 Nov 1;177(9):5852-60. doi: 10.4049/jimmunol.177.9.5852.
7
Treatment of Intestinal Inflammation With Epicutaneous Immunotherapy Requires TGF-β and IL-10 but Not Foxp3 Tregs.经皮免疫治疗肠道炎症需要 TGF-β 和 IL-10，但不需要 Foxp3 Tregs。
Front Immunol. 2021 Feb 26;12:637630. doi: 10.3389/fimmu.2021.637630. eCollection 2021.
8
Wiskott-Aldrich syndrome protein deficiency in innate immune cells leads to mucosal immune dysregulation and colitis in mice.先天免疫细胞中威特克-奥尔德里奇综合征蛋白缺陷导致小鼠黏膜免疫失调和结肠炎。
Gastroenterology. 2012 Sep;143(3):719-729.e2. doi: 10.1053/j.gastro.2012.06.008. Epub 2012 Jun 15.
9
Lactobacillus acidophilus Improves Intestinal Inflammation in an Acute Colitis Mouse Model by Regulation of Th17 and Treg Cell Balance and Fibrosis Development.嗜酸乳杆菌通过调节Th17和Treg细胞平衡及纤维化发展改善急性结肠炎小鼠模型的肠道炎症。
J Med Food. 2018 Mar;21(3):215-224. doi: 10.1089/jmf.2017.3990. Epub 2018 Jan 16.
10
Targeting aberrant colon cancer-specific DNA methylation with lipoteichoic acid-deficient Lactobacillus acidophilus.用缺乏脂磷壁酸的嗜酸乳杆菌靶向异常的结肠癌特异性 DNA 甲基化。
Gut Microbes. 2013 Jan-Feb;4(1):84-8. doi: 10.4161/gmic.22822. Epub 2012 Nov 8.
引用本文的文献
1
The role of probiotics in promoting systemic immune tolerance in systemic lupus erythematosus.益生菌在促进系统性红斑狼疮患者全身免疫耐受中的作用。
Gut Pathog. 2025 Jun 17;17(1):45. doi: 10.1186/s13099-025-00702-7.
2
Gut commensal Bifidobacterium-derived extracellular vesicles modulate the therapeutic effects of anti-PD-1 in lung cancer.肠道共生双歧杆菌衍生的细胞外囊泡调节抗PD-1在肺癌中的治疗效果。
Nat Commun. 2025 Apr 12;16(1):3500. doi: 10.1038/s41467-025-58553-4.
3
Comparative Genomic and Functional Evaluations of Newly Isolated from Korean Traditional Fermented Foods.从韩国传统发酵食品中新分离菌株的比较基因组学和功能评估。
Foods. 2020 Dec 4;9(12):1805. doi: 10.3390/foods9121805.
4
Effects and immune responses of probiotic treatment in ruminants.反刍动物益生菌治疗的效果及免疫反应。
Vet Immunol Immunopathol. 2019 Feb;208:58-66. doi: 10.1016/j.vetimm.2018.12.006. Epub 2019 Jan 6.
5
Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice.植物乳杆菌菌株OLL2712培养条件对小鼠免疫细胞以及肥胖和2型糖尿病小鼠抗炎活性的影响
Appl Environ Microbiol. 2017 Mar 17;83(7). doi: 10.1128/AEM.03001-16. Print 2017 Apr 1.
6
Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria.革兰氏阳性菌中脂磷壁酸的结构多样性及生物学意义：聚焦有益的益生菌乳酸菌
Biosci Microbiota Food Health. 2016;35(4):147-161. doi: 10.12938/bmfh.2016-006. Epub 2016 Jun 9.
7
Advancing the use of Lactobacillus acidophilus surface layer protein A for the treatment of intestinal disorders in humans.推进嗜酸乳杆菌表层蛋白A在人类肠道疾病治疗中的应用。
Gut Microbes. 2015;6(6):392-7. doi: 10.1080/19490976.2015.1107697.
8
Human dendritic cell DC-SIGN and TLR-2 mediate complementary immune regulatory activities in response to Lactobacillus rhamnosus JB-1.人类树突状细胞DC-SIGN和TLR-2介导对鼠李糖乳杆菌JB-1的互补性免疫调节活性。
PLoS One. 2015 Mar 27;10(3):e0120261. doi: 10.1371/journal.pone.0120261. eCollection 2015.
9
Microbial activation of gut dendritic cells and the control of mucosal immunity.肠道树突状细胞的微生物激活与黏膜免疫的调控。
J Interferon Cytokine Res. 2013 Nov;33(11):619-31. doi: 10.1089/jir.2013.0046. Epub 2013 Aug 20.
10
Role of commensal and probiotic bacteria in human health: a focus on inflammatory bowel disease.共生菌和益生菌在人类健康中的作用：以炎症性肠病为重点。
Microb Cell Fact. 2013 Jul 23;12:71. doi: 10.1186/1475-2859-12-71.
本文引用的文献
1
Lipoteichoic acid-deficient Lactobacillus acidophilus regulates downstream signals.缺失脂磷壁酸的嗜酸乳杆菌调节下游信号。
Immunotherapy. 2011 Mar;3(3):337-47. doi: 10.2217/imt.10.119.
2
Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid.脂磷壁酸缺陷型嗜酸乳杆菌对诱导性结肠炎症的调控作用。
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4623-30. doi: 10.1073/pnas.1005066107. Epub 2011 Jan 31.
3
Intestinal inflammation abrogates the tolerogenic properties of MLN CD103+ dendritic cells.肠道炎症会破坏 MLN CD103+树突状细胞的耐受原性。
Eur J Immunol. 2010 Jul;40(7):1877-83. doi: 10.1002/eji.200939957.
4
Intestinal CD103+, but not CX3CR1+, antigen sampling cells migrate in lymph and serve classical dendritic cell functions.肠道 CD103+，但不是 CX3CR1+，抗原采样细胞在淋巴中迁移，并发挥经典树突状细胞的功能。
J Exp Med. 2009 Dec 21;206(13):3101-14. doi: 10.1084/jem.20091925. Epub 2009 Dec 14.
5
Induction of intestinal Th17 cells by segmented filamentous bacteria.分节丝状菌诱导肠道Th17细胞
Cell. 2009 Oct 30;139(3):485-98. doi: 10.1016/j.cell.2009.09.033.
6
Interleukin 10 acts on regulatory T cells to maintain expression of the transcription factor Foxp3 and suppressive function in mice with colitis.白细胞介素10作用于调节性T细胞，以维持结肠炎小鼠中转录因子Foxp3的表达和抑制功能。
Nat Immunol. 2009 Nov;10(11):1178-84. doi: 10.1038/ni.1791. Epub 2009 Sep 27.
7
Origin of the lamina propria dendritic cell network.固有层树突状细胞网络的起源。
Immunity. 2009 Sep 18;31(3):513-25. doi: 10.1016/j.immuni.2009.08.010. Epub 2009 Sep 10.
8
Balancing inflammation and tolerance in vivo through dendritic cells by the commensal Candida albicans.共生白色念珠菌通过树突状细胞在体内平衡炎症与耐受性。
Mucosal Immunol. 2009 Jul;2(4):362-74. doi: 10.1038/mi.2009.17. Epub 2009 May 6.
9
Intestinal epithelial cells promote colitis-protective regulatory T-cell differentiation through dendritic cell conditioning.肠道上皮细胞通过调节树突状细胞促进具有结肠炎保护作用的调节性T细胞分化。
Mucosal Immunol. 2009 Jul;2(4):340-50. doi: 10.1038/mi.2009.13. Epub 2009 Apr 22.
10
Recent progress in understanding the phenotype and function of intestinal dendritic cells and macrophages.肠道树突状细胞和巨噬细胞的表型与功能研究的最新进展
Mucosal Immunol. 2008 Nov;1(6):460-9. doi: 10.1038/mi.2008.61. Epub 2008 Sep 17.
Zotero 插件