Milk Science Research Institute, Megmilk Snow Brand Co., Ltd, Kawagoe, Saitama, Japan.
Anim Sci J. 2012 Feb;83(2):178-83. doi: 10.1111/j.1740-0929.2011.00940.x. Epub 2011 Aug 19.
We previously found that dietary sphingomyelin (SPM) concentrate from bovine milk improved epidermal function. In this study, we investigated the dosage of dietary SPM concentrate from bovine milk in relation to the improvement of epidermal function. Thirteen-week-old hairless male mice were separated into four experimental groups, each fed one of four types of experimental diet: the control group, the low SPM group, the medium SPM group and the high SPM group. The mice were each fed the experimental diet for 6 weeks. The stratum corneum hydration and the transepidermal water loss (TEWL) were measured using a Corneometer and a Tewameter at 3 weeks and 6 weeks. After the feeding period, ceramides in the stratum corneum were analyzed. We found that the stratum corneum hydration in all the SPM groups was significantly higher than that in the control group, whereas TEWL in all the SPM groups was significantly lower than that in the control group. Ceramides increased significantly in mice fed the medium SPM diet and statistically tended to increase in mice fed the high SPM diet. Our results indicate that a daily intake of 17 mg SPM concentrate is enough to improve epidermal function in hairless mice.
我们之前发现牛乳来源的鞘磷脂（SPM）浓缩物可以改善表皮功能。在这项研究中，我们研究了牛乳来源的饮食 SPM 浓缩物的剂量与改善表皮功能之间的关系。将 13 周龄无毛雄性小鼠分为四组实验，每组喂食四种实验饮食中的一种：对照组、低 SPM 组、中 SPM 组和高 SPM 组。将这些小鼠分别喂食实验饮食 6 周。在第 3 周和第 6 周，使用 Corneometer 和 Tewameter 测量角质层含水量和经皮水分流失（TEWL）。喂养期结束后，分析角质层中的神经酰胺。我们发现，所有 SPM 组的角质层含水量均明显高于对照组，而所有 SPM 组的 TEWL 均明显低于对照组。中 SPM 饮食组的神经酰胺显著增加，高 SPM 饮食组的神经酰胺有统计学趋势增加。我们的结果表明，每日摄入 17mg 的 SPM 浓缩物足以改善无毛小鼠的表皮功能。
