[微生物脂肪酶的特性。1.脂肪酶活性的测定]

[The characterization of microbial lipases. 1. The determination of lipase activity].

作者信息

Bariszlovich M, Meusel D, Tülsner M

机构信息

Wissenschaftsbereich Lebensmittelchemie Humboldt-Universität zu, Berlin, DDR.

出版信息

Nahrung. 1990;34(8):701-17. doi: 10.1002/food.19900340811.

DOI:10.1002/food.19900340811

PMID:2233988

Abstract

In the selection of an appropriate method for activity determination of lipases existing technical equipment, kind of enzymes, number of samples investigated (e.g. in routine analysis), and expected sensitivity range have to be taken into account. Titrimetric methods and above all copper salt methods with their high detection sensitivity are the most suitable procedures for activity determination of lipases used in laboratories and institutions without equipment for radiochemical analysis.

摘要

在选择测定脂肪酶活性的合适方法时，必须考虑现有技术设备、酶的种类、所研究样品的数量（如在常规分析中）以及预期的灵敏度范围。滴定法，尤其是具有高检测灵敏度的铜盐法，是在没有放射化学分析设备的实验室和机构中测定脂肪酶活性的最合适方法。