Institute for Biochemistry, Zülpicher Str. 47, 50674 Cologne, Germany.
Mol Microbiol. 2012 Apr;84(1):105-16. doi: 10.1111/j.1365-2958.2012.08011.x. Epub 2012 Feb 28.
In eukaryotic and prokaryotic cells the establishment and maintenance of cell polarity is essential for numerous biological processes. In some bacterial species, the chromosome origins have been identified as molecular markers of cell polarity and polar chromosome anchoring factors have been identified, for example in Caulobacter crescentus. Although speculated, polar chromosome tethering factors have not been identified for Actinobacteria, to date. Here, using a minimal synthetic Escherichia coli system, biochemical and in vivo experiments, we provide evidence that Corynebacterium glutamicum cells tether the chromosome origins at the cell poles through direct physical interactions between the ParB-parS chromosomal centromere and the apical growth determinant DivIVA. The interaction between ParB and DivIVA proteins was also shown for other members of the Actinobacteria phylum, including Mycobacterium tuberculosis and Streptomyces coelicolor.
在真核细胞和原核细胞中，细胞极性的建立和维持对于许多生物过程都是至关重要的。在一些细菌物种中，染色体起源已被确定为细胞极性的分子标记，并且已经鉴定出了极性染色体锚定因子，例如在新月柄杆菌中。尽管有推测，但迄今为止，尚未为放线菌鉴定出极性染色体系绳因子。在这里，我们使用最小的合成大肠杆菌系统、生化和体内实验，提供了证据表明谷氨酸棒状杆菌细胞通过 ParB-parS 染色体着丝粒与顶端生长决定因素 DivIVA 之间的直接物理相互作用将染色体起源系绳在细胞的两极。ParB 和 DivIVA 蛋白之间的相互作用也在放线菌门的其他成员中得到了证实，包括结核分枝杆菌和链霉菌。
