Parwin Shabnam, Srivastava Preeti
Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology, Delhi, New Delhi, India.
Int Microbiol. 2025 Aug 8. doi: 10.1007/s10123-025-00702-2.
Unlike other Actinobacteria, Rhodococcus erythropolis PR4 contains three different copies of divIVA genes. The three divIVA genes show about 22-38% similarity with each other. Deletion mutants of the divIVA genes resulted in altered morphology: elongated cells in △divIVA-2 and short rod-shaped cells in △divIVA-1 and △divIVA-3. The expression of the divIVA-2 gene was found to be higher as compared to the divIVA-1 and divIVA-3 genes in wild-type cells. The subcellular localisation studies revealed that the three different DivIVA proteins are spatially present at different regions of cell space. Our results suggest that an interplay of the three DivIVA proteins plays a role in cell shape maintenance in R. erythropolis PR4. Bioinformatics analysis and interactome study show that amongst the three copies of divIVA genes, the second copy, i.e., divIVA-2 gene, might have a more governing function in the cell division process as compared to the other two copies.
与其他放线菌不同，红平红球菌PR4含有三个不同拷贝的divIVA基因。这三个divIVA基因彼此之间的相似度约为22%-38%。divIVA基因的缺失突变体导致形态改变：△divIVA-2中的细胞伸长，△divIVA-1和△divIVA-3中的细胞呈短杆状。在野生型细胞中，发现divIVA-2基因的表达高于divIVA-1和divIVA-3基因。亚细胞定位研究表明，三种不同的DivIVA蛋白在细胞空间的不同区域呈空间分布。我们的结果表明，三种DivIVA蛋白之间的相互作用在红平红球菌PR4的细胞形态维持中起作用。生物信息学分析和相互作用组研究表明，在divIVA基因的三个拷贝中，第二个拷贝即divIVA-2基因在细胞分裂过程中可能比其他两个拷贝具有更重要的调控功能。
