Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang-gun, Busan, Republic of Korea.
Fish Shellfish Immunol. 2012 May;32(5):939-44. doi: 10.1016/j.fsi.2012.02.003. Epub 2012 Feb 9.
Galectin-9 is a b-galactoside-binding lectin that regulates many cellular functions, ranging from cell adhesion to pathogen recognition. We isolated and characterized the cDNA of tandem-repeat galectin-9 (RuGlec9) from the Korean rose bitterling (Rhodeus uyekii), an endemic Korean fish belonging to the Acheilognathinae subfamily of the Cyprinidae family. RuGlec9 cDNA is 1486 bp long and encodes a polypeptide of 323 amino acids containing two carbohydrate-recognition domains connected by a linker peptide. The deduced amino acid sequence of RuGlec9 shows 45-84% amino acid sequence identity to other galectin-9 sequences, including those from mammals and fish. RuGlec9 appeared in a large cluster with other galectin-9 sequences from fish and is more closely related to galectin-9 from Danio rerio than to those of other fish and mammals. RuGlec9 mRNA was expressed highly in the testis, spleen, intestine, stomach, and liver, and moderately in the brain, kidney, ovary, and gills of normal Korean rose bitterling. RuGlec9 mRNA expression in the spleen was increased by lipopolysaccharide. These results suggest that RuGlec9 plays a role in innate immunity in Korean rose bitterling.
半乳糖凝集素-9 是一种β-半乳糖苷结合凝集素，可调节多种细胞功能，从细胞黏附到病原体识别。我们从韩国玫瑰鲫（Rhodeus uyekii）中分离并鉴定了串联重复半乳糖凝集素-9（RuGlec9）的 cDNA，韩国玫瑰鲫是一种韩国特有的鱼类，属于鲤科的 Acheilognathinae 亚科。RuGlec9 cDNA 长 1486bp，编码一个 323 个氨基酸的多肽，其中包含两个通过连接肽连接的碳水化合物识别结构域。RuGlec9 的推导氨基酸序列与其他半乳糖凝集素-9 序列（包括来自哺乳动物和鱼类的序列）具有 45-84%的氨基酸序列同一性。RuGlec9 与鱼类的其他半乳糖凝集素-9 序列一起出现在一个大簇中，与斑马鱼的半乳糖凝集素-9 比与其他鱼类和哺乳动物的半乳糖凝集素-9 更密切相关。在正常的韩国玫瑰鲫中，RuGlec9 mRNA 在睾丸、脾脏、肠道、胃和肝脏中高度表达，在大脑、肾脏、卵巢和鳃中中度表达。脾脏中的 RuGlec9 mRNA 表达受脂多糖诱导增加。这些结果表明，RuGlec9 在韩国玫瑰鲫的固有免疫中发挥作用。
