Cho Hyun Kook, Kong Hee Jeong, Moon Ji-Young, Kim Jin-Do, Kim Dong-Gyun, Kim Woo-Jin, Nam Bo-Hye, Kim Young-Ok, Kim Hyung Soo, An Cheul Min, Kim Bong-Seok
Biotechnology Research Division, National Fisheries Research and Development Institute, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan, 619-705, Republic of Korea.
Mol Biol Rep. 2014;41(4):2363-70. doi: 10.1007/s11033-014-3090-0. Epub 2014 Jan 14.
Peroxiredoxins (Prxs), also known as natural killer cell enhancing factors in fish, role as antioxidant proteins and participate in a variety of biological processes, including H2O2-mediated cell signaling, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 1 cDNA from the Korean rose bitterling Rhodeus uyekii, and designated it RuPrx 1. The RuPrx 1 cDNA encodes a 197-amino-acid polypeptide that belongs to the class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced RuPrx 1 protein shows strong homology (77.38-92.89 %) with Prx 1 proteins from other species, including fish, amphibians, and mammals, and it is most closely related to rainbow smelt Prx 1. RuPrx 1 mRNA was ubiquitously detected in all tested tissues and its expression was comparatively high in the brain, intestine, kidney, liver, ovary, stomach, and testis. Expression of RuPrx 1 mRNA in liver peaked 3 h post-infection with Aeromonas hydrophila and decreased 24 h post-infection while the expression in intestine decreased 24 h post-infection. These results suggest that RuPrx 1 is conserved through evolution and may play roles similar to its mammalian counterparts.
过氧化物酶（Prxs），在鱼类中也被称为自然杀伤细胞增强因子，作为抗氧化蛋白发挥作用，并参与多种生物过程，包括过氧化氢（H2O2）介导的细胞信号传导、分子伴侣功能以及线粒体功能。在本研究中，我们从韩国玫瑰无须鲃（Rhodeus uyekii）中分离并鉴定了一个Prx 1 cDNA，并将其命名为RuPrx 1。RuPrx 1 cDNA编码一个197个氨基酸的多肽，属于典型的2 - 半胱氨酸过氧化物酶类，包含过氧化物酶和还原半胱氨酸。推导的RuPrx 1蛋白与其他物种（包括鱼类、两栖动物和哺乳动物）的Prx 1蛋白具有高度同源性（77.38 - 92.89%），并且与彩虹胡瓜鱼Prx 1关系最为密切。在所有测试组织中均普遍检测到RuPrx 1 mRNA，其在脑、肠、肾、肝、卵巢、胃和睾丸中的表达相对较高。用嗜水气单胞菌感染后，肝脏中RuPrx 1 mRNA的表达在感染后3小时达到峰值，在感染后24小时下降，而肠中的表达在感染后24小时下降。这些结果表明RuPrx 1在进化过程中是保守的，并且可能发挥与其哺乳动物对应物相似的作用。
