Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
Dev Comp Immunol. 2020 May;106:103607. doi: 10.1016/j.dci.2020.103607. Epub 2020 Jan 2.
We isolated and characterised a cDNA encoding the aspartic protease cathepsin E (CTSE) in Korean rose bitterling, Rhodeus uyekii. The full-length Rhodeus uyekii CTSE (RuCTSE) cDNA (1396 bp) contains an open reading frame of 1218 bp, encoding 405 amino acids. Alignment of multiple CTSE protein sequences revealed that two of the aspartyl protease active site residues and a disulphide bond were well-conserved among the other CTSE sequences. Phylogenetic analysis revealed that RuCTSE is most closely related to freshwater fish cathepsin E. RuCTSE is widely expressed in the liver, spleen, ovary, testis, brain, eye, intestine, muscle, fin, stomach, and kidney. This first report of teleost CTSE will provide important information related to the identification of other cathepsin E genes in various fish species and will serve as a useful molecular tool to help clarify biological activities in other teleosts.
我们从韩国鱒鱼中分离并鉴定了编码天冬氨酸蛋白酶组织蛋白酶 E (CTSE)的 cDNA。韩国鱒鱼 CTSE (RuCTSE) cDNA 的全长为 1396 bp，包含一个 1218 bp 的开放阅读框，编码 405 个氨基酸。对多个 CTSE 蛋白序列的比对表明，两个天冬氨酸蛋白酶活性位点残基和一个二硫键在其他 CTSE 序列中得到了很好的保守。系统发育分析表明，RuCTSE 与淡水鱼组织蛋白酶 E 最为密切相关。RuCTSE 在肝脏、脾脏、卵巢、睾丸、大脑、眼睛、肠道、肌肉、鳍、胃和肾脏中广泛表达。这是首次报道硬骨鱼 CTSE，将为鉴定各种鱼类中的其他组织蛋白酶 E 基因提供重要信息，并将作为有用的分子工具，有助于阐明其他硬骨鱼的生物学活性。
