Department of Biotechnology, Yung-Ta Institute of Technology and Commerce, Pingtung, Taiwan.
Indian J Pharmacol. 2012 Jan;44(1):26-30. doi: 10.4103/0253-7613.91862.
Broussonetia papyrifera is used as a traditional medicine to treat few diseases. However, the antiinflammatory effect of B. papyrifera stem bark has not been evaluated. The aim of this study is to investigate the effects of n-hexane fraction from methanol extract of B. papyrifera stem bark on lipopolysaccharide (LPS)-stimulated inflammation using RAW 264.7 cells.
Methanol extract was obtained from B. papyrifera stem bark and its sequential fractions (hexane, dichloromathane, ethyl acetate, butanol, and aqueous) were obtained by extraction in solvents with increasing polarity and were examined in RAW 264.7 cells.
The secretion profiles of pro-inflammatory parameters, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were found to be significantly reduced in 10-80 μg/ml dose ranges of n-hexane fraction (BP-H) from methanol extract of B. papyrifera stem bark. The expressions of inducible NO synthase (iNOS) was also significantly inhibited by BP-H. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that BP-H treatment decreased LPS-induced iNOS mRNA expression in RAW 264.7 cells.
The results suggest that the B. papyrifera stem bark has anti-inflammatory activity which inhibits the NO production and proinflammatory cytokines in RAW 264.7 cells. B. papyrifera stem bark might act as a potential therapeutic agent for inflammatory diseases.
构树白皮被用作治疗少数疾病的传统药物。然而，构树白皮的抗炎作用尚未得到评估。本研究旨在通过 RAW 264.7 细胞来研究甲醇提取物的正己烷部分对脂多糖（LPS）刺激炎症的影响。
从构树白皮中获得甲醇提取物，并用有机溶剂按极性递增顺序萃取，获得其连续馏分（正己烷、二氯甲烷、乙酸乙酯、正丁醇和水），并在 RAW 264.7 细胞中进行检验。
在 10-80μg/ml 剂量范围内，构树白皮甲醇提取物的正己烷部分（BP-H）可显著降低促炎参数包括一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的分泌谱。诱导型一氧化氮合酶（iNOS）的表达也被 BP-H 显著抑制。逆转录-聚合酶链反应（RT-PCR）分析表明，BP-H 处理可降低 LPS 诱导的 RAW 264.7 细胞中 iNOS mRNA 的表达。
研究结果表明，构树白皮具有抗炎活性，可抑制 RAW 264.7 细胞中 NO 的产生和促炎细胞因子。构树白皮可能是一种治疗炎症性疾病的潜在治疗剂。
