Hwang Jung-Hee, Lee Byung Mu
Division of Toxicology, School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-Do, South Korea.
J Toxicol Environ Health A. 2007 Mar 1;70(5):393-407. doi: 10.1080/10937400600882871.
For medical, pharmacological, and cosmetic reasons, the demand for effective and safe depigmentating agents has increased. In this study, 101 plant extracts (methanol or water extracts) were screened for their inhibitory activities against tyrosinase, (L-3, 4,-dihydroxyphenylalanine) L-DOPA oxidation, and melanin biosynthesis in B16 mouse melanoma cells. Of the extracts examined, 31 showed over 50% inhibition of mushroom tyrosinase at a concentration of 666 microg/Ml, and 11 inhibited L-DOPA auto-oxidation at this concentration. In particular, extracts of Broussonetia kazinoki var. humilis (leaves and stems), Broussonetia papyrifera (leaves and bark), Cornus officinalis (fruit), Rhus javanica (gallnut), and Pinus densiflora (leaves) inhibited both tyrosinase activity and L-DOPA oxidation in a concentration-dependent manner. Seventeen plant extracts that inhibited tyrosinase were further tested for their inhibitory effects on melanogenesis. In B16 mouse melanoma cells, extracts of Acorus gramineus, Capsella bursa-pastoris, Morus bombycis, Perilla frutescens var. crispa, Quercus dentate (bark), Rhus javanica (gallnut), Schizopepon bryoniaefolius, or Sophora flavescens markedly inhibited (>50%) melanin synthesis at 50 microg/Ml. These plants represent a potential source of novel whitening agents for ultraviolet (UV)-sensitive skin.
出于医学、药理学和美容方面的原因，对有效且安全的色素脱失剂的需求日益增加。在本研究中，对101种植物提取物（甲醇提取物或水提取物）进行了筛选，考察它们对B16小鼠黑色素瘤细胞中酪氨酸酶、L-3,4-二羟基苯丙氨酸（L-DOPA）氧化以及黑色素生物合成的抑制活性。在所检测的提取物中，31种在浓度为666微克/毫升时对蘑菇酪氨酸酶的抑制率超过50%，11种在此浓度下抑制L-DOPA的自动氧化。特别地，小构树变种矮构树（叶和茎）、构树（叶和树皮）、山茱萸（果实）、盐肤木（五倍子）和赤松（叶）的提取物以浓度依赖方式同时抑制酪氨酸酶活性和L-DOPA氧化。对17种抑制酪氨酸酶的植物提取物进一步测试其对黑色素生成的抑制作用。在B16小鼠黑色素瘤细胞中，石菖蒲、荠菜、桑蚕、皱叶紫苏、槲树（树皮）、盐肤木（五倍子）、裂瓜或苦参的提取物在50微克/毫升时显著抑制（>50%）黑色素合成。这些植物是对紫外线（UV）敏感皮肤新型美白剂的潜在来源。
