Departamento de Química Analítica. Facultad de Ciencias Químicas. Universidad Complutense de Madrid. E-28040 Madrid, Spain; E-Mails:
Sensors (Basel). 2009;9(7):5503-20. doi: 10.3390/s90705503. Epub 2009 Jul 13.
The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered.
病原体微生物的检测和识别仍然依赖于传统的培养技术,这些技术不适合现场监测。因此,该领域的一个重大研究挑战是需要开发快速、可靠、特异和灵敏的方法,以低成本检测这些细菌。此外,人们对生物芯片用于大规模筛选分析的兴趣日益浓厚,这意味着需要提高微型化程度、缩短分析时间和降低成本,并实现多分析物检测,这已成为当今的一个关键挑战。本文综述了基于电化学传感器与微阵列和其他平台相结合的多重食源性病原体微生物检测方法。这些装置通常涉及抗体-抗原和 DNA 杂交的特异性相互作用,尽管也考虑了其他方法,如氧消耗监测。