Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
ACS Sens. 2021 May 28;6(5):1717-1730. doi: 10.1021/acssensors.1c00481. Epub 2021 May 6.
Bacterial foodborne pathogens cause millions of illnesses each year and disproportionately impact those in developing countries. To combat these diseases and their spread, effective monitoring of foodborne pathogens is needed. Technologies to detect these microbes must be deployable at the point-of-contamination, often in nonideal environments. Electrochemical sensors are uniquely suited for field-deployable monitoring, as they are quantitative, rapid, and do not require expensive instrumentation. When combined with the inherent recognition capabilities of biomolecules, electrochemistry is unmatched for quantitative biological measurements with minimal equipment requirements. This Review is centered on recent advances in electrochemical sensors for the detection of bacterial foodborne pathogens with a specific emphasis on field-deployable platforms, as this is a key requirement of any technology that could effectively halt the spread of foodborne diseases. Innovative electrochemical sensing strategies are highlighted that demonstrate the ability of these technologies to achieve high sensitivity and large detection ranges with rapid readout. Sensing strategies are categorized on the basis of whether they incorporate biological pretreatments or biorecognition elements, and their key advantages and disadvantages are summarized. As this class of sensors continues to mature, methods to incorporate device specificity and to detect targets from complex solutions will enable the translation of these platforms from laboratory prototypes to real-world implementation.
细菌食源性致病菌每年导致数百万人患病，且在发展中国家的影响更为严重。为了应对这些疾病及其传播，需要对食源性致病菌进行有效的监测。用于检测这些微生物的技术必须能够在污染点部署，通常是在不理想的环境中。电化学传感器非常适合现场部署监测，因为它们是定量的、快速的，并且不需要昂贵的仪器。当与生物分子的固有识别能力相结合时，电化学在最小设备要求下进行定量生物测量是无与伦比的。本综述主要介绍了用于检测细菌食源性致病菌的电化学传感器的最新进展，特别强调了现场部署平台，因为这是任何能够有效阻止食源性疾病传播的技术的关键要求。突出了创新性的电化学传感策略，这些策略展示了这些技术实现高灵敏度和大检测范围的快速读数的能力。根据是否包含生物预处理或生物识别元件对传感策略进行分类，并总结了它们的主要优缺点。随着这类传感器的不断成熟，将器件特异性和从复杂溶液中检测目标的方法相结合，将使这些平台从实验室原型转化为实际应用。
