血红扇头蜱和边缘璃眼蜱对绵羊巴贝斯虫的经卵传播
Transovarial Transmission of Babesia ovis by Rhipicephalus sanguineus and Hyalomma marginatum.
作者信息
Razmi Ghr, Nouroozi E
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University, P.O.Box:91775-1793, Mashhad, Iran.
出版信息
Iran J Parasitol. 2010 Sep;5(3):35-9.
BACKGROUND
Rhipicephalus sanguineus and Hyalomma marginatum are the most common species in sheep herds in Northeast of Iran. There is preliminary evidence that these species may be the vectors of Babesia ovis in Iran. We carried out two experiments in Mashhad area, Khorasan Razavi Province to determine whether B. ovis could be transovarially transmitted by R. sanguineus and H. marginatum.
METHODS
In experiment 1, adults of laboratory reared H. marginatum and R.sanguineus were infected with B. ovis isolated from naturally infected sheep in Mashhad area by feeding the ticks on the sheep inoculated intravenously by infected blood samples. The inoculated sheep showed clinical signs with parasitaemia while the adult ticks were engorging on them. The engorged females were collected and kept at 28°C and 85% relative humidity in incubator. Then, larval, nymphal and adult stages derived from engorged females were used to infest the clean sheep. In experiment 2, two splenectomized sheep were infested only with the same adult ticks of two species.
RESULTS
Examination of smears and PCR of blood samples to detect of B. ovis in infested sheep in two experiments were negative.
CONCLUSION
It seems that R. sanguineus and H. marginatum can not transovarially transmit B. ovis in sheep.
背景
血红扇头蜱和边缘璃眼蜱是伊朗东北部绵羊群中最常见的蜱种。有初步证据表明，这些蜱种可能是伊朗绵羊巴贝斯虫的传播媒介。我们在霍拉桑拉扎维省马什哈德地区进行了两项实验，以确定血红扇头蜱和边缘璃眼蜱是否能经卵传播绵羊巴贝斯虫。
方法
在实验1中，通过让实验室饲养的边缘璃眼蜱和血红扇头蜱成虫吸食经感染血液样本静脉接种的绵羊，使其感染从马什哈德地区自然感染绵羊中分离出的绵羊巴贝斯虫。接种的绵羊在成虫吸食血液时出现临床症状并伴有寄生虫血症。收集饱血雌蜱并置于28°C、相对湿度85%的培养箱中。然后，用饱血雌蜱产出的幼虫、若虫和成虫感染健康绵羊。在实验2中，两只脾切除的绵羊仅感染这两种蜱的成虫。
结果
在两项实验中，对感染绵羊的血液样本进行涂片检查和聚合酶链反应以检测绵羊巴贝斯虫，结果均为阴性。
结论
看来血红扇头蜱和边缘璃眼蜱不能经卵传播绵羊巴贝斯虫。
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