Rahimi H, Sadjjadi Sm, Sarkari B
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Parasitol. 2011 Mar;6(1):12-9.
The aim of this study was to assess the performance of Antigen B (AgB) isolated from different Echinococcusgranulosus intermediate hosts and from different cyst locations for immunodiagnosis of human cystic echinococcosis (CE).
Hydatid cyst fluids were collected from lung and liver cysts of sheep, liver cysts of goats, lung cysts of camels and cattle, and liver cysts of human. AgB was purified from each of these hydatid cysts fluids. Serum samples obtained from 47 pathologically confirmed cases of CE along with 30 sera samples from non-CE patients and 40 sera from healthy individuals were tested by ELISA using AgB prepared from different hosts or cyst locations.
The highest sensitivity (97.8%) for diagnosis of CE was seen with AgB prepared from human liver cysts. This maximal sensitivity was followed by AgB isolated from those of sheep liver and lung cysts. The least sensitivity was found with AgB prepared from bovine lung cysts. The highest specificities (97.1%) were observed with AgB isolated from human liver cysts followed by those of sheep and goat liver cysts while the lowest specificity was seen with AgB isolated from bovine lung cysts. In view of the specificities and sensitivities of the different AgB, the best validity was found for AgB prepared from human liver cysts while the least validity was found with AgB prepared from bovine lung cysts.
For any AgB-based tests, obtaining of the antigen from one of these sources will significantly increase the diagnostic sensitivity and specificity of the assay.
本研究的目的是评估从不同细粒棘球绦虫中间宿主以及不同囊肿部位分离出的抗原B（AgB）用于人类囊型棘球蚴病（CE）免疫诊断的性能。
从绵羊的肺和肝囊肿、山羊的肝囊肿、骆驼和牛的肺囊肿以及人类的肝囊肿中收集包虫囊肿液。从这些包虫囊肿液中分别纯化AgB。使用从不同宿主或囊肿部位制备的AgB，通过酶联免疫吸附测定（ELISA）对47例经病理确诊的CE患者的血清样本以及30例非CE患者的血清样本和40例健康个体的血清进行检测。
用人肝囊肿制备的AgB对CE诊断的敏感性最高（97.8%）。其次是从绵羊肝和肺囊肿中分离出的AgB。用牛肺囊肿制备的AgB敏感性最低。从人肝囊肿中分离出的AgB特异性最高（97.1%），其次是绵羊和山羊肝囊肿，而从牛肺囊肿中分离出的AgB特异性最低。鉴于不同AgB的特异性和敏感性，用人肝囊肿制备的AgB有效性最佳，而用牛肺囊肿制备的AgB有效性最差。
对于任何基于AgB的检测，从这些来源之一获取抗原将显著提高检测的诊断敏感性和特异性。
