埃及细粒棘球绦虫分离株中抗原B的遗传变异性
Genetic variability of antigen B among Echinococcus granulosus Egyptian isolates.
作者信息
Tawfeek Gihan M, Elwakil Hala S, Awad Nabil S, El-Hoseiny Laila, Thabet Hala S, Sarhan Rania M, Darweesh Samar K, Anwar Wagida A
机构信息
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
出版信息
Korean J Parasitol. 2009 Sep;47(3):259-64. doi: 10.3347/kjp.2009.47.3.259. Epub 2009 Aug 28.
Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.
利用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）和DNA测序技术，对20株埃及细粒棘球绦虫分离株中编码抗原B2基因（AgB2）的遗传多态性进行了研究。收集并使用了来自不同宿主来源（人类、骆驼、猪和绵羊）的5株分离株。在用AluI进行限制性酶切后，每个宿主组的所有检测分离株均呈现出非常相似的PCR-RFLP模式，绵羊和人类分离株的基因大小约为140 bp和240 bp，猪和骆驼分离株的基因大小约为150 bp和250 bp。在用EcoRI孵育所有研究分离株后，未获得消化模式。这些结果显示出组内高度同质性。DNA序列分析突出表明，感染人类的菌株与感染绵羊的分离株具有100%的同一性，与感染猪和骆驼的分离株分别具有96%和99%的同一性。
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