Mahdhi A, Hmila Z, Behi A, Bakhrouf A
Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP). Faculty of Pharmacy, University of Monastir, 5000. Tunisia.
Iran J Microbiol. 2011 Sep;3(3):129-34.
Probiotics are live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance, producing metabolites which inhibit the colonization or growth of other microorganisms or by competing with them for resources such as nutrients or space. The aim of this study was to investigate the probiotic properties of Candida famata and Geobacillus thermoleovorans.
In this study, yeast and bacterial strains isolated from pure oil waste were identified using Api 50 CHB and Api Candida Systems and their probiotic properties were studied through antimicrobial activity, biofilm production, adherence assay and enzymatic characterization.
According to biochemical analyses, these strains corresponded to Geobacillus thermoleovorans and Candida famata. Antagonism assay results showed that the tested strains have an inhibitory effect against tested pathogenic bacteria. The yeast Candida famata was unable to produce biofilm on Congo Red Agar (CRA), while the bacterial strain was a slime producer. Adherence assays to abiotic surfaces revealed that the investigated strains were fairly adhesive to polystyrene with values ranging from 0.18 to 0.34 at 595 nm. The enzymatic characterization revealed that the tested strains expressed enzymes such as phosphatase alkaline, esterase lipase (C8), amylase, lipase, lecitenase and caseinase. The obtained results may allow the isolated strains to be considered as having the potential to be candidate probiotics.
益生菌是活的微生物饲料添加剂，通过改善宿主动物的肠道微生物平衡、产生抑制其他微生物定殖或生长的代谢产物，或与它们竞争营养物质或空间等资源，从而对宿主动物产生有益影响。本研究的目的是探究法马假丝酵母和嗜热栖热放线菌的益生菌特性。
在本研究中，使用Api 50 CHB和Api假丝酵母系统对从纯油废料中分离出的酵母和细菌菌株进行鉴定，并通过抗菌活性、生物膜形成、黏附试验和酶学特性研究其益生菌特性。
根据生化分析，这些菌株分别对应嗜热栖热放线菌和法马假丝酵母。拮抗试验结果表明，受试菌株对受试病原菌具有抑制作用。法马假丝酵母在刚果红琼脂（CRA）上无法形成生物膜，而该细菌菌株是黏液产生菌。对非生物表面的黏附试验表明，受试菌株对聚苯乙烯具有相当强的黏附性，在595 nm处的值范围为0.18至0.34。酶学特性表明，受试菌株表达碱性磷酸酶、酯酶脂肪酶（C8）、淀粉酶、脂肪酶、卵磷脂酶和酪蛋白酶等酶。所得结果可能使分离出的菌株被认为有潜力成为候选益生菌。
