Al-Seraih Alaa, Flahaut Christophe, Krier François, Cudennec Benoit, Drider Djamel
Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université Lille 1 - Sciences et Technologies, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université d'Artois, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
Probiotics Antimicrob Proteins. 2015 Dec;7(4):233-41. doi: 10.1007/s12602-015-9201-y.
We studied here the yeast content of poultry feces, collected randomly from a French farm located in the north of the country. Thus, 81 yeast colonies were isolated and clustered into 22 distinct groups using the rep-PCR method. A single colony was taken from each group and identified using biochemical (ID 32C system) and molecular (sequencing of the D1/D2 domain of 26S rDNA and ITS1-5.8-ITS2 rDNA region) methods. Both methods led to the identification of Candida famata species. One isolate of C. famata strains, named strain Y5, was further studied for its cytotoxicity, adhesion, and surface properties, hemolytic activity, and its survival in simulated gastric and intestine environments. The data obtained advocate the probiotic potential of this isolate.
我们在此研究了从法国北部一个农场随机采集的家禽粪便中的酵母含量。由此，使用rep-PCR方法分离出81个酵母菌落并聚为22个不同的组。从每组中选取一个菌落，采用生化方法（ID 32C系统）和分子方法（26S rDNA的D1/D2结构域及ITS1-5.8-ITS2 rDNA区域测序）进行鉴定。两种方法均鉴定出法塔假丝酵母。对一株法塔假丝酵母菌株（命名为Y5菌株）进一步研究了其细胞毒性、黏附性、表面特性、溶血活性以及在模拟胃和肠道环境中的存活情况。所获数据表明该分离株具有益生菌潜力。
