Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Mol Biol Rep. 2012 May;39(5):6337-42. doi: 10.1007/s11033-012-1455-9. Epub 2012 Feb 18.
The black carp Mylopharyngodon piceus (Cyprinidae), native to eastern Asian, is a large, commercially important fish, and has been introduced to many other countries for variable reasons. In this study, the complete mitochondrial genome sequences from three specimens of black carp were first determined and were used to evaluate the sister relationship between black carp and grass carp (Ctenopharyngodon idellus). Two individuals had a mitogenome of 16,609 bp, while the other was 16,611 bp in length. Similar to most vertebrates, the black carp contains the same gene order and an identical number of genes or regions, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 putative control region. Phylogenetic analyses using three different computational algorithms (Maximum Parsimony, Maximum Likelihood, and Bayesian analysis) revealed two distinct clades in subfamily Leuciscinae. However, the sister taxonomic relationship of black carp and grass carp was not observed using sequences of nearly complete mitochondrial genomes, which suggests more nuclear gene markers are needed to resolve the phylogenetic relationship between black carp and grass carp.
原产于东亚的鲤鱼科大口黑鲈（Mylopharyngodon piceus）是一种大型的、具有商业重要性的鱼类，已被引入许多其他国家，原因各不相同。在这项研究中，首次测定了三个大口黑鲈标本的完整线粒体基因组序列，并利用这些序列评估了大口黑鲈与草鱼（Ctenopharyngodon idellus）的姐妹关系。两个个体的线粒体基因组长度为 16609bp，而另一个个体的长度为 16611bp。与大多数脊椎动物一样，大口黑鲈的基因排列顺序和基因或区域数量相同，包括 13 个蛋白质编码基因、2 个 rRNA 基因、22 个 tRNA 基因和 1 个假定的控制区。使用三种不同的计算算法（最大简约法、最大似然法和贝叶斯分析）进行的系统发育分析显示，在 Leuciscinae 亚科中存在两个不同的分支。然而，利用近乎完整的线粒体基因组序列并未观察到大口黑鲈和草鱼的姐妹分类关系，这表明需要更多的核基因标记来解决大口黑鲈和草鱼之间的系统发育关系。
