Department of General Surgery, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
Ann Surg Oncol. 2012 Sep;19(9):3081-8. doi: 10.1245/s10434-012-2279-5. Epub 2012 Feb 17.
Period 3 (PER3), a circadian regulation protein, influences cell cycle, growth, and differentiation. The aim of the present study was to determine whether PER3 expression is associated with colon cancer incidence and progression.
PER3 expression was analyzed in the normal and cancerous tissues from patients with colon cancer by establishing a long serial analysis of gene expression (SAGE) database as well as by real-time PCR and immunohistochemistry.
As compared with normal tissue, a 2.8-fold decrease in PER3 mRNA levels in colon cancerous tissue was observed. Real-time PCR analysis revealed that PER3 mRNA levels in tumor tissues were lower than in normal tissues (P < 0.001) in both patients with colon tumor and those with rectal tumor. In addition, PER3 expression was related to multiple clinicopathologic factors, including tumor location, differentiation, and stage. Furthermore, the incidence of death was higher in subjects with PER3-negative tumors (P = 0.025); the estimated overall survival time was 71.5 ± 2.2 months and 58.6 ± 5.0 months in subjects with PER3-positive and PER3-negative tumors, respectively (P = 0.020).
PER3 may play a role in colon cancer progression.
PER3（周期 3）是一种生物钟调节蛋白，它影响细胞周期、生长和分化。本研究旨在确定 PER3 的表达是否与结肠癌的发生和进展有关。
通过建立长序列基因表达分析（SAGE）数据库以及实时 PCR 和免疫组织化学分析，检测 PER3 在结肠癌患者正常和癌变组织中的表达。
与正常组织相比，结肠癌组织中 PER3 mRNA 水平下降了 2.8 倍。实时 PCR 分析显示，无论是结肠癌患者还是直肠癌患者，肿瘤组织中的 PER3 mRNA 水平均低于正常组织（P<0.001）。此外，PER3 的表达与多种临床病理因素有关，包括肿瘤位置、分化和分期。此外，PER3 阴性肿瘤患者的死亡率更高（P=0.025）；PER3 阳性和 PER3 阴性肿瘤患者的估计总生存期分别为 71.5±2.2 个月和 58.6±5.0 个月（P=0.020）。
PER3 可能在结肠癌的进展中发挥作用。
