Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, People's Republic of China.
Luminescence. 2013 Jan-Feb;28(1):16-22. doi: 10.1002/bio.1388. Epub 2012 Feb 21.
We report for the first time that the sensitivity of the luminol-hypochlorite chemiluminescence (CL) reaction was enhanced approximately 10 times by the addition of phloxine B. The maximum wavelength of CL emission shifted from 431 to 595 nm in the absence and presence, respectively, of phloxine B, suggesting that an efficient chemiluminescence resonance energy transfer occurred between a luminol donor and a phloxine B acceptor in the luminol-hypochlorite-phloxine B system. Based on this observation, a simple, rapid and sensitive microflow injection CL method, using a microchip with spiral channel configurations, was developed for the determination of hypochlorite. Under optimized conditions, a linear calibration curve (R(2) = 0.9944) over the range 0.1-10.0 µmol/L was obtained, with a detection limit of 0.025 µmol/L (S:N = 3). The relative standard deviation (RSD) was found to be 4.2% (n = 10) for 2.5 µmol/L hypochlorite. The sample consumption was only 2 μL, with a sample throughput of 90/h. The method has been used for determining trace amounts of hypochlorite in water samples with satisfactory results.
我们首次报道，在加入荧光素 B 后，鲁米诺-次氯酸盐化学发光（CL）反应的灵敏度提高了约 10 倍。CL 发射的最大波长从 431nm 分别转移到 595nm，在没有和存在荧光素 B 的情况下，表明在鲁米诺供体和荧光素 B 受体之间发生了有效的化学发光共振能量转移在鲁米诺-次氯酸盐-荧光素 B 体系中。基于这一观察结果，使用具有螺旋通道配置的微芯片，开发了一种简单、快速和灵敏的微流动注射 CL 方法，用于测定次氯酸盐。在优化条件下，在 0.1-10.0µmol/L 范围内获得了线性校准曲线（R(2) = 0.9944），检测限为 0.025µmol/L（S:N = 3）。对于 2.5µmol/L 的次氯酸盐，相对标准偏差（RSD）为 4.2%（n = 10）。样品消耗量仅为 2μL，样品通量为 90/h。该方法已用于测定水样中的痕量次氯酸盐，结果令人满意。
