通过体外位点特异性重组进行埃坡霉素生物合成基因簇的串联组装。

Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

Sci Rep. 2011;1:141. doi: 10.1038/srep00141. Epub 2011 Nov 3.

We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.

我们描述了一种基于位点特异性重组的串联组装（SSRTA）方法，用于在合成生物学中重建生物部件。该系统由链霉菌噬菌体 φBT1 整合酶催化，该酶属于大丝氨酸重组酶亚家族。这种一步法高效、准确，能够按预定顺序在体外连接多个 DNA 分子。因此，它可能在构建代谢途径和遗传网络方面有应用。