Li Jianhua, Meng Hailin, Wang Yong
Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Bioengineering Research Center, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458, China.
Synth Syst Biotechnol. 2016 Oct 31;1(4):221-229. doi: 10.1016/j.synbio.2016.08.002. eCollection 2016 Dec.
Natural products (NPs) continue to play a pivotal role in drug discovery programs. The rapid development of synthetic biology has conferred the strategies of NPs production. Synthetic biology is a new engineering discipline that aims to produce desirable products by rationally programming the biological parts and manipulating the pathways. However, there is still a challenge for integrating a heterologous pathway in chassis cells for overproduction purpose due to the limited characterized parts, modules incompatibility, and cell tolerance towards product. Enormous endeavors have been taken for mentioned issues. Herein, in this review, the progresses in naturally discovering novel biological parts and rational design of synthetic biological parts are reviewed, combining with the advanced assembly technologies, pathway engineering, and pathway optimization in global network guidance. The future perspectives are also presented.
天然产物（NPs）在药物研发项目中持续发挥着关键作用。合成生物学的快速发展赋予了天然产物生产的策略。合成生物学是一门新兴的工程学科，旨在通过合理编程生物元件和操纵代谢途径来生产所需产品。然而，由于已表征元件有限、模块不兼容性以及细胞对产物的耐受性等问题，在底盘细胞中整合异源途径以实现过量生产仍面临挑战。针对上述问题已付出巨大努力。在此综述中，结合先进的组装技术、途径工程以及全局网络指导下的途径优化，对天然发现新型生物元件和合成生物元件的合理设计方面的进展进行了综述。同时也给出了未来展望。
