State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Tai'an Subcenter of National Wheat Improvement Center, Agronomy College, Shandong Agricultural University, Taian 271018, Shandong, China.
J Biosci. 2012 Mar;37(1):149-55. doi: 10.1007/s12038-011-9175-1.
Novel dwarfing germplasms and dwarfing genes are valuable for the wheat breeding. A novel semi-dwarf line, 31505-1, with reduced height compared with its common wheat parent, was derived from a cross between common wheat and Thinopyrum ponticum. Cytological studies demonstrated that 31505-1 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) analysis showed that 31505-1 had no large Th. ponticum chromosome fragments. Fluorescence in situ hybridization (FISH) results revealed the absence of a pAs1 hybridization band on 2DL chromosome of 31505-1. Two SSR markers (Xwmc41 and Xcfd168) and two STS markers (Xmag4059 and Xmag3596), which were located on 2D chromosome, amplified unique bands of Th. Ponticum in 31505-1. These revealed presence of an introgressed Th. ponticum segment in 2DL chromosome of dwarf line 31505-1, although the alien segment could not be detected by GISH.
新型矮秆种质资源和矮秆基因对小麦育种具有重要价值。新型半矮秆系 31505-1 与普通小麦亲本相比，株高降低。该系是由普通小麦与节节麦杂交育成。细胞学研究表明，31505-1 含有 42 条染色体，在减数分裂中期 I 形成 21 条二价体。基因组原位杂交（GISH）分析表明，31505-1 中没有大的节节麦染色体片段。荧光原位杂交（FISH）结果显示，31505-1 的 2DL 染色体上没有 pAs1 杂交带。两个 SSR 标记（Xwmc41 和 Xcfd168）和两个 STS 标记（Xmag4059 和 Xmag3596）位于 2D 染色体上，在 31505-1 中扩增出节节麦的独特条带。这表明矮秆系 31505-1 的 2DL 染色体上存在一个导入的节节麦片段，尽管 GISH 未能检测到该外源片段。
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