State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Genome. 2017 Oct;60(10):860-867. doi: 10.1139/gen-2017-0099. Epub 2017 Jul 31.
Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of J genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7J (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.
节节麦（Th. ponticum）（2n = 10x = 70）是一种具有优异抗性和应激耐受性的重要育种材料。本研究通过细胞学、形态学、基因组原位杂交（GISH）、荧光原位杂交（FISH）、表达序列标签（EST）和基于 PCR 的标志独特基因（PLUG）标记分析，对衍生系 CH1113-B13-1-1-2-1（CH1113-B13）进行了表征。GISH 分析表明，CH1113-B13 含有 20 对普通小麦染色体和 1 对 J 基因组染色体。利用 7 个 EST 和 7 个 PLUG 标记对节节麦进行连锁分析表明，这对异源染色体属于第七同源群。缺体-四体分析和 FISH 分析表明，CH1113-B13 中不存在小麦 7B 染色体；因此，CH1113-B13 被鉴定为 7J（7B）代换系。最后，成体 CH1113-B13 对小麦条锈病表现出免疫性。因此，该代换系是小麦育种的一种很有前途的种质资源。
