Leelavatcharamas V, Emery A N, al-Rubeai M
BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
Cytotechnology. 1994;15(1-3):65-71. doi: 10.1007/BF00762380.
The relationship between growth and interferon-gamma (IFN-gamma) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The Ks and mu max-values were found to be 0.771% (v/v) serum and 0.031 h-1 respectively. The average specific IFN-gamma production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-gamma production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.
通过改变胎牛血清（FCS）浓度，研究了重组细胞系CHO 320中生长与干扰素-γ（IFN-γ）产生之间的关系。比生长速率随初始FCS浓度变化，其变化方式可用莫诺德模型很好地拟合。发现Ks和μmax值分别为0.771%（v/v）血清和0.031 h-1。指数生长期的平均比IFN-γ产生速率随FCS浓度增加而增加。除延迟期外，在培养的所有阶段均发现比产生速率与比生长速率之间具有良好的相关性，并且清楚地表明IFN-γ产生与生长相关。葡萄糖和谷氨酰胺的比利用率与比生长速率呈负相关。
