在恒化培养的低血清含量培养基中从人早幼粒细胞中经济地生产抗微生物因子。
The economic production of anti-microbial factor from human promyelocytes in low serum containing medium under chemostat cultivation.
机构信息
Department of Food Engineering, Kangwon National University, 200-701, Chunchon, Korea.
出版信息
Cytotechnology. 1995 Jan;19(1):73-8. doi: 10.1007/BF00749757.
It proves that a purifed Anti-Microbial Factor (AMF) from human promyelocytes has strong activity on Gram(-) and Gram(+) bacteria, showing 0.5 (μg/ml) of Minimal Bacterical Concentration (MBC) on bothE. coli andS. aureus. For mass production of AMF, chemostat cultivation is recommended to accumulate cells out of the reactor since it is an intracellular protein and its system requires only 1% serum in the medium. Its production process proves to be closely growth-related. 1.7×10(-8) (μg/viable cell/day) of maximum specific AMF production rate is estimated at 0.026 h(-1) of dilution rate, maintaining 6×10(6) (viable cell/ml). Ca. 300 (mg/ml) of crude AMF can be obtained for 50 days of continuous cultivation under optimal conditions. The cell growth reaches relatively fast steady state.
它证明了从人早幼粒细胞中提取的一种纯化的抗微生物因子（AMF）对革兰氏阴性菌和革兰氏阳性菌都具有很强的活性，对大肠杆菌和金黄色葡萄球菌的最小细菌浓度（MBC）均为 0.5（μg/ml）。为了大规模生产 AMF，建议使用恒化器培养来积累细胞，因为它是一种细胞内蛋白，其系统只需要培养基中 1%的血清。其生产过程被证明与生长密切相关。在最佳条件下，连续培养 50 天，可获得约 300（mg/ml）的粗 AMF。细胞生长达到相对较快的稳定状态。在稀释率为 0.026 h(-1)时，估计最大比生产速率为 1.7×10(-8)（μg/活细胞/天），维持 6×10(6)（活细胞/ml）。当采用最佳条件进行连续培养 50 天时，可获得约 300（mg/ml）的粗 AMF。细胞生长达到相对较快的稳定状态。在稀释率为 0.026 h(-1)时，估计最大比生产速率为 1.7×10(-8)（μg/活细胞/天），维持 6×10(6)（活细胞/ml）。
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