GIBCO-BRL, Life Technologies, Inc., 3175 Staley Road, 14072, Grand Island, NY, USA.
Cytotechnology. 1996 Jan;22(1-3):255-61. doi: 10.1007/BF00353946.
We designed an Integrated Media Preparation System (IMPS) for continuous, on-line preparation of cell culture media and delivery to intermediate storage vessels or directly to a bioreactor. Key components of the IMPS include: a high precision, continuous fluid mixing device; formulation-specific liquid medium concentrates; validated process controls and membrane filtration; and automated dispensing into large volume flexible plastic containers. The IMPS system is designed to produce sterile, single-strength liquid medium from common raw materials at a delivery rate of 1000-3000 liters per hour and will manufacture homogenous batches from several thousand liters to over 60,000 liters. Fortified nutrient media prepared from multi-component 50X concentrates have been demonstrated to accelerate bioreactor seed chains, increase product yield, and reduce the overall manufacturing cost of nutrient medium. A productivity matrix will analyze the fully-loaded costs and contrast alternative methods for media preparation against projected biological yield.
我们设计了一种集成式媒体制备系统（IMPS），用于连续、在线制备细胞培养基，并将其输送到中间储存容器或直接输送到生物反应器中。IMPS 的关键组件包括：高精度、连续流体混合装置；特定配方的液体培养基浓缩物；经过验证的过程控制和膜过滤；以及自动分配到大容量柔性塑料容器中。IMPS 系统旨在以每小时 1000-3000 升的输送速度，使用普通原料生产无菌、单强度液体培养基，并可生产从几千升到超过 60000 升的均质批次。由多组分 50X 浓缩物制备的强化营养培养基已被证明可加速生物反应器种子链、提高产品产量，并降低营养培养基的整体制造成本。生产力矩阵将分析满载成本，并对比替代的培养基制备方法与预期的生物产量。
