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β-珠蛋白基因座激活区域：组织、结构和功能的保守性

Beta-globin locus activation regions: conservation of organization, structure, and function.

作者信息

Li Q L, Zhou B, Powers P, Enver T, Stamatoyannopoulos G

机构信息

Shanghai Institute of Biochemistry, Chinese Academy of Sciences.

出版信息

Proc Natl Acad Sci U S A. 1990 Nov;87(21):8207-11. doi: 10.1073/pnas.87.21.8207.

DOI:10.1073/pnas.87.21.8207
PMID:2236034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC54924/
Abstract

The human beta-globin locus activation region (LAR) comprises four erythroid-specific DNase I hypersensitive sites (I-IV) thought to be largely responsible for activating the beta-globin domain and facilitating high-level erythroid-specific globin gene expression. We identified the goat beta-globin LAR, determined 10.2 kilobases of its sequence, and demonstrated its function in transgenic mice. The human and goat LARs share 6.5 kilobases of homologous sequences that are as highly conserved as the epsilon-globin gene promoters. Furthermore, the overall spatial organization of the two LARs has been conserved. These results suggest that the functionally relevant regions of the LAR are large and that in addition to their primary structure, the spatial relationship of the conserved elements is important for LAR function.

摘要

人类β-珠蛋白基因座激活区（LAR）由四个红系特异性DNase I高敏位点（I-IV）组成，这些位点被认为在很大程度上负责激活β-珠蛋白结构域并促进高水平的红系特异性珠蛋白基因表达。我们鉴定了山羊β-珠蛋白LAR，测定了其10.2千碱基的序列，并在转基因小鼠中证明了其功能。人类和山羊的LAR有6.5千碱基的同源序列，其保守程度与ε-珠蛋白基因启动子一样高。此外，两个LAR的整体空间组织也得以保留。这些结果表明，LAR的功能相关区域很大，并且除了其一级结构外，保守元件的空间关系对LAR功能也很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/8bcb1e7d1afb/pnas01046-0033-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/6224f45a7be6/pnas01046-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/d66d7372bfdd/pnas01046-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/b845568411ff/pnas01046-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/604db01f166d/pnas01046-0033-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/65f6a08730ac/pnas01046-0033-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/8bcb1e7d1afb/pnas01046-0033-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/6224f45a7be6/pnas01046-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/d66d7372bfdd/pnas01046-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/b845568411ff/pnas01046-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/604db01f166d/pnas01046-0033-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/65f6a08730ac/pnas01046-0033-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f28/54924/8bcb1e7d1afb/pnas01046-0033-e.jpg

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本文引用的文献

1
Triplication of a four-gene set during evolution of the goat beta-globin locus produced three genes now expressed differentially during development.在山羊β-珠蛋白基因座进化过程中，一个四基因组合的三倍化产生了三个在发育过程中差异表达的基因。
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6589-93. doi: 10.1073/pnas.81.21.6589.
2
Nucleotide sequence of 16-kilobase pairs of DNA 5' to the human epsilon-globin gene.人类ε-珠蛋白基因5'端16千碱基对DNA的核苷酸序列。
J Biol Chem. 1985 Dec 5;260(28):14901-10.
3
The "beta-like-globin" gene domain in human erythroid cells.
5'HS3缺失突变揭示的β-珠蛋白基因座控制区（LCR）的协同和累加特性：对LCR染色质结构的影响
Mol Cell Biol. 2005 Aug;25(16):7033-41. doi: 10.1128/MCB.25.16.7033-7041.2005.
4
Locus control regions.位点控制区
Blood. 2002 Nov 1;100(9):3077-86. doi: 10.1182/blood-2002-04-1104.
5
Developmental specificity of the interaction between the locus control region and embryonic or fetal globin genes in transgenic mice with an HS3 core deletion.在具有HS3核心缺失的转基因小鼠中，基因座控制区与胚胎或胎儿珠蛋白基因之间相互作用的发育特异性。
Mol Cell Biol. 1998 Jul;18(7):4188-96. doi: 10.1128/MCB.18.7.4188.
6
The activation domain of the enhancer binding protein p45NF-E2 interacts with TAFII130 and mediates long-range activation of the alpha- and beta-globin gene loci in an erythroid cell line.增强子结合蛋白p45NF-E2的激活结构域与TAFII130相互作用，并介导红细胞系中α和β珠蛋白基因位点的远程激活。
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10051-6. doi: 10.1073/pnas.94.19.10051.
7
Multiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells.红系细胞中β-珠蛋白基因表达需要p45 NF-E2的多个区域。
Nucleic Acids Res. 1997 Jun 15;25(12):2509-15. doi: 10.1093/nar/25.12.2509.
8
Sequences within and flanking hypersensitive sites 3 and 2 of the beta-globin locus control region required for synergistic versus additive interaction with the epsilon-globin gene promoter.β-珠蛋白基因座控制区超敏位点3和2内部及侧翼的序列，是与ε-珠蛋白基因启动子协同相互作用而非加性相互作用所必需的。
Nucleic Acids Res. 1996 Nov 1;24(21):4327-35. doi: 10.1093/nar/24.21.4327.
9
Effect of deletion of 5'HS3 or 5'HS2 of the human beta-globin locus control region on the developmental regulation of globin gene expression in beta-globin locus yeast artificial chromosome transgenic mice.人β-珠蛋白基因座控制区5'HS3或5'HS2缺失对β-珠蛋白基因座酵母人工染色体转基因小鼠中珠蛋白基因表达发育调控的影响
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6605-9. doi: 10.1073/pnas.93.13.6605.
10
Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3.对含有β-珠蛋白基因座控制区5'超敏位点3靶向缺失的小鼠的分析。
Mol Cell Biol. 1996 Jun;16(6):2906-12. doi: 10.1128/MCB.16.6.2906.
人类红细胞中的“类β-珠蛋白”基因结构域。
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8. doi: 10.1073/pnas.82.19.6384.
4
Developmental regulation of a cloned adult beta-globin gene in transgenic mice.转基因小鼠中克隆的成人β-珠蛋白基因的发育调控
Nature. 1985;315(6017):338-40. doi: 10.1038/315338a0.
5
Regulated expression of human A gamma-, beta-, and hybrid gamma beta-globin genes in transgenic mice: manipulation of the developmental expression patterns.人γA、β和γβ杂交珠蛋白基因在转基因小鼠中的调控表达：发育表达模式的操纵
Cell. 1986 Jul 4;46(1):89-94. doi: 10.1016/0092-8674(86)90862-7.
6
Position-independent, high-level expression of the human beta-globin gene in transgenic mice.人β-珠蛋白基因在转基因小鼠中与位置无关的高水平表达。
Cell. 1987 Dec 24;51(6):975-85. doi: 10.1016/0092-8674(87)90584-8.
7
A developmentally stable chromatin structure in the human beta-globin gene cluster.人类β-珠蛋白基因簇中一种发育稳定的染色质结构。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.
8
Nucleotide sequence of the BALB/c mouse beta-globin complex.
J Mol Biol. 1989 Jan 5;205(1):41-62. doi: 10.1016/0022-2836(89)90363-x.
9
The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.β-珠蛋白显性控制区以组织特异性方式激活同源和异源启动子。
Cell. 1989 Mar 24;56(6):969-77. doi: 10.1016/0092-8674(89)90630-2.
10
A dominant control region from the human beta-globin locus conferring integration site-independent gene expression.来自人类β-珠蛋白基因座的一个显性控制区域，可实现不依赖整合位点的基因表达。
Nature. 1989 Mar 23;338(6213):352-5. doi: 10.1038/338352a0.