• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人类β-珠蛋白基因簇中一种发育稳定的染色质结构。

A developmentally stable chromatin structure in the human beta-globin gene cluster.

作者信息

Forrester W C, Thompson C, Elder J T, Groudine M

出版信息

Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.

DOI:10.1073/pnas.83.5.1359
PMID:3456593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC323075/
Abstract

The DNase I-hypersensitive sites in the human embryonic beta-globin gene region have been mapped in erythroid-enriched fractions of disaggregated fetal livers, in adult nucleated red blood cells, and in fetal brain tissue. Our analysis of a region extending 11 kilobases (kb) 5' of the epsilon-globin gene reveals many minor nuclease-hypersensitive sites and one major site located 6.1 kb upstream of the epsilon-globin gene. All of these hypersensitive sites are erythroid-specific, and the major site is stable throughout erythroid development. As assayed by nuclear runoff transcription, little or no epsilon-globin gene expression is detectable in fetal or adult erythroid cells. Thus, the presence of the major hypersensitive site 5' of the epsilon-globin gene in both fetal and adult erythroid cells demonstrates that this site is not specifically correlated with transcription of the gene or with a particular stage of development. Rather, this site may reflect an early event in erythroid differentiation. In addition, DNase I has been used to probe the overall sensitivity of epsilon-globin chromatin in fetal erythroid cells. Our findings indicate that the epsilon-globin gene as well as the other genes in the beta-globin cluster reside within the chromatin domain that is more DNase I-sensitive than "bulk" chromatin.

摘要

人类胚胎β-珠蛋白基因区域中的脱氧核糖核酸酶I超敏位点已在解离的胎儿肝脏富含红细胞的组分、成体有核红细胞以及胎儿脑组织中进行了定位。我们对ε-珠蛋白基因5'端延伸11千碱基（kb）的区域进行分析，发现了许多微小的核酸酶超敏位点以及一个位于ε-珠蛋白基因上游6.1 kb处的主要位点。所有这些超敏位点都是红细胞特异性的，并且该主要位点在整个红细胞发育过程中都是稳定的。通过核转录分析，在胎儿或成体红细胞中几乎检测不到ε-珠蛋白基因的表达。因此，在胎儿和成体红细胞中ε-珠蛋白基因5'端存在主要超敏位点表明，该位点与基因转录或特定发育阶段没有特异性关联。相反，该位点可能反映了红细胞分化中的早期事件。此外，脱氧核糖核酸酶I已被用于探测胎儿红细胞中ε-珠蛋白染色质的整体敏感性。我们的研究结果表明，ε-珠蛋白基因以及β-珠蛋白基因簇中的其他基因位于比“整体”染色质对脱氧核糖核酸酶I更敏感的染色质结构域内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/f04866047022/pnas00309-0201-h.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/15ee1eb3ad07/pnas00309-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/c4f9b09de239/pnas00309-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/160adb333a7f/pnas00309-0200-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/2ca7fd1ba041/pnas00309-0200-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/075b80e2a936/pnas00309-0200-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/8400f6366b69/pnas00309-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/95a220ffc0ce/pnas00309-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/fa17df4a4633/pnas00309-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/b8632f4aec74/pnas00309-0201-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/5a42772db770/pnas00309-0201-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/b3777258912d/pnas00309-0201-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/95e29af57e6f/pnas00309-0201-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/f04866047022/pnas00309-0201-h.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/15ee1eb3ad07/pnas00309-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/c4f9b09de239/pnas00309-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/160adb333a7f/pnas00309-0200-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/2ca7fd1ba041/pnas00309-0200-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/075b80e2a936/pnas00309-0200-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/8400f6366b69/pnas00309-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/95a220ffc0ce/pnas00309-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/fa17df4a4633/pnas00309-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/b8632f4aec74/pnas00309-0201-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/5a42772db770/pnas00309-0201-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/b3777258912d/pnas00309-0201-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/95e29af57e6f/pnas00309-0201-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e9/323075/f04866047022/pnas00309-0201-h.jpg

相似文献

1
A developmentally stable chromatin structure in the human beta-globin gene cluster.人类β-珠蛋白基因簇中一种发育稳定的染色质结构。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.
2
Correlation between patterns of DNase I-hypersensitive sites and upstream promoter activity of the human epsilon-globin gene at different stages of erythroid development.红细胞生成不同阶段人ε-珠蛋白基因的DNase I超敏位点模式与上游启动子活性之间的相关性。
Mol Cell Biol. 1990 Mar;10(3):1199-208. doi: 10.1128/mcb.10.3.1199-1208.1990.
3
Autonomous, erythroid-specific DNase I hypersensitive site formed by human beta-globin locus control region (LCR) 5' HS 2 in transgenic mice.人β-珠蛋白基因座控制区（LCR）5' HS 2在转基因小鼠中形成的自主性、红系特异性脱氧核糖核酸酶I超敏感位点。
Dev Biol. 1995 Jun;169(2):728-32. doi: 10.1006/dbio.1995.1182.
4
Mapping of DNase I-hypersensitive sites in the upstream DNA of human embryonic epsilon-globin gene in K562 leukemia cells.K562白血病细胞中人胚胎ε-珠蛋白基因上游DNA中DNase I超敏位点的定位
Proc Natl Acad Sci U S A. 1984 May;81(9):2718-22. doi: 10.1073/pnas.81.9.2718.
5
Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.人类β-珠蛋白结构域两侧的红系特异性核酸酶超敏位点。
Mol Cell Biol. 1990 Aug;10(8):4324-33. doi: 10.1128/mcb.10.8.4324-4333.1990.
6
Developmentally regulated and erythroid-specific expression of the human embryonic beta-globin gene in transgenic mice.人类胚胎β-珠蛋白基因在转基因小鼠中的发育调控及红系特异性表达。
Nucleic Acids Res. 1990 Sep 25;18(18):5465-72. doi: 10.1093/nar/18.18.5465.
7
Developmental regulation of topoisomerase II sites and DNase I-hypersensitive sites in the chicken beta-globin locus.鸡β-珠蛋白基因座中拓扑异构酶II位点和DNase I超敏位点的发育调控
Mol Cell Biol. 1990 Jun;10(6):2774-86. doi: 10.1128/mcb.10.6.2774-2786.1990.
8
Chromatin structure and developmental expression of the human alpha-globin cluster.人类α-珠蛋白基因簇的染色质结构与发育表达
Mol Cell Biol. 1986 Apr;6(4):1108-16. doi: 10.1128/mcb.6.4.1108-1116.1986.
9
Evidence for a locus activation region: the formation of developmentally stable hypersensitive sites in globin-expressing hybrids.基因座激活区域的证据：在表达珠蛋白的杂种中形成发育稳定的超敏位点。
Nucleic Acids Res. 1987 Dec 23;15(24):10159-77. doi: 10.1093/nar/15.24.10159.
10
The "beta-like-globin" gene domain in human erythroid cells.人类红细胞中的“类β-珠蛋白”基因结构域。
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8. doi: 10.1073/pnas.82.19.6384.

引用本文的文献

1
Transcriptional Repressor BCL11A in Erythroid Cells.红细胞中的转录抑制因子 BCL11A。
Adv Exp Med Biol. 2024;1459:199-215. doi: 10.1007/978-3-031-62731-6_9.
2
CLOCI: unveiling cryptic fungal gene clusters with generalized detection.CLOCI：利用广义检测揭示隐匿真菌基因簇。
Nucleic Acids Res. 2024 Sep 9;52(16):e75. doi: 10.1093/nar/gkae625.
3
RS12574989 and haplotype associated with α/β-chain imbalance and population HbA2 reduction.与 α/β 链失衡和人群 HbA2 降低相关的 RS12574989 及其单倍型。

本文引用的文献

1
Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.对人类ε-珠蛋白基因5'侧翼区域存在的两个Alu家族重复序列之一进行选择性体外转录。
Nucleic Acids Res. 1981 Dec 21;9(24):6709-22. doi: 10.1093/nar/9.24.6709.
2
Human globin psi B2 is not a globin-related sequence.人类珠蛋白ψ B2不是与珠蛋白相关的序列。
Nucleic Acids Res. 1982 Dec 11;10(23):7809-18. doi: 10.1093/nar/10.23.7809.
3
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
BMC Med Genomics. 2022 Aug 15;15(1):179. doi: 10.1186/s12920-022-01333-6.
4
Targeting Genetic Modifiers of HBG Gene Expression in Sickle Cell Disease: The miRNA Option.靶向镰状细胞病 HBG 基因表达的遗传修饰物：miRNA 方案。
Mol Diagn Ther. 2022 Sep;26(5):497-509. doi: 10.1007/s40291-022-00589-z. Epub 2022 May 12.
5
Recent Approaches for Manipulating Globin Gene Expression in Treating Hemoglobinopathies.治疗血红蛋白病中调控珠蛋白基因表达的最新方法
Front Genome Ed. 2021 Aug 2;3:618111. doi: 10.3389/fgeed.2021.618111. eCollection 2021.
6
Deficiencies in the DNA Binding Protein ARID3a Alter Chromatin Structures Important for Early Human Erythropoiesis.DNA 结合蛋白 ARID3a 的缺乏会改变早期人类红细胞生成过程中重要的染色质结构。
Immunohorizons. 2021 Oct 18;5(10):802-817. doi: 10.4049/immunohorizons.2100083.
7
Exploring epigenetic and microRNA approaches for γ-globin gene regulation.探索γ-珠蛋白基因调控的表观遗传和 microRNA 方法。
Exp Biol Med (Maywood). 2021 Nov;246(22):2347-2357. doi: 10.1177/15353702211028195. Epub 2021 Jul 22.
8
Patterns of variation in cis-regulatory regions: examining evidence of purifying selection.顺式调控区变异模式：检验纯化选择的证据。
BMC Genomics. 2018 Jan 26;19(1):95. doi: 10.1186/s12864-017-4422-y.
9
Regulatory Enhancer-Core-Promoter Communication via Transcription Factors and Cofactors.通过转录因子和辅助因子实现的调控增强子-核心启动子通讯
Trends Genet. 2016 Dec;32(12):801-814. doi: 10.1016/j.tig.2016.10.003. Epub 2016 Nov 2.
10
Current and future alternative therapies for beta-thalassemia major.重型β地中海贫血的现有及未来替代疗法
Biomed J. 2016 Feb;39(1):24-38. doi: 10.1016/j.bj.2015.10.001. Epub 2016 Apr 6.
染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
Nature. 1980 Aug 28;286(5776):854-60. doi: 10.1038/286854a0.
4
The molecular genetics of human hemoglobins.人类血红蛋白的分子遗传学。
Annu Rev Genet. 1980;14:145-78. doi: 10.1146/annurev.ge.14.120180.001045.
5
Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy-chain locus.免疫球蛋白重链基因座中的增强子对易位的人类c-myc基因的激活作用。
Nature. 1984;307(5949):334-40. doi: 10.1038/307334a0.
6
Molecular mechanisms of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts.人类血红蛋白转换的分子机制：胚胎、胎儿及成人成红细胞中珠蛋白基因的选择性低甲基化与表达
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6907-11. doi: 10.1073/pnas.80.22.6907.
7
Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos.源自K562细胞和正常人胚胎中mRNA帽位点上游的ε珠蛋白基因转录本。
Nucleic Acids Res. 1982 Sep 11;10(17):5133-47. doi: 10.1093/nar/10.17.5133.
8
Propagation of globin DNAase I-hypersensitive sites in absence of factors required for induction: a possible mechanism for determination.在缺乏诱导所需因子的情况下珠蛋白DNA酶I超敏位点的传播：一种可能的决定机制。
Cell. 1982 Aug;30(1):131-9. doi: 10.1016/0092-8674(82)90019-8.
9
The primary structure of the human epsilon-globin gene.人类ε-珠蛋白基因的一级结构。
Cell. 1980 Oct;21(3):621-6. doi: 10.1016/0092-8674(80)90425-0.
10
The 5' flanking region of human epsilon-globin gene.人类ε-珠蛋白基因的5'侧翼区。
Nucleic Acids Res. 1980 Oct 10;8(19):4393-404. doi: 10.1093/nar/8.19.4393.