Tuan D, Solomon W, Li Q, London I M
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8. doi: 10.1073/pnas.82.19.6384.
We have mapped the distribution of the major and minor DNase I-hypersensitive sites in the human "beta-like-globin" gene domain. The minor DNase I-hypersensitive sites map close to the 5' end of each of the beta-like-globin genes. Their presence is specifically associated with the transcription of the immediate downstream beta-like-globin genes. The major DNase I-hypersensitive sites map in what appear to be the 5' and 3' boundary areas of the human beta-like-globin gene domain, a region estimated to span at least 90 kilobases of DNA. These major sites are present in various erythroid cells, which express predominantly either the embryonic, the fetal, or the adult beta-like-globin genes, and seem to be involved in defining the active beta-like-globin genes domain in cells of erythroid lineage. The four major DNase I-hypersensitive sites in the 5' boundary area, when correlated with sequencing data, are shown to be located in DNA regions containing enhancer core-like sequences and alternating purine and pyrimidine bases.
我们已经绘制了人类“类β珠蛋白”基因区域中主要和次要DNase I超敏位点的分布图。次要DNase I超敏位点位于每个类β珠蛋白基因的5'端附近。它们的存在与紧邻下游的类β珠蛋白基因的转录特别相关。主要DNase I超敏位点位于人类类β珠蛋白基因区域的5'和3'边界区域，该区域估计跨越至少90千碱基的DNA。这些主要位点存在于各种红细胞中，这些红细胞主要表达胚胎、胎儿或成人的类β珠蛋白基因，并且似乎参与了确定红细胞谱系细胞中的活性类β珠蛋白基因区域。5'边界区域中的四个主要DNase I超敏位点与测序数据相关时，显示位于含有增强子核心样序列以及嘌呤和嘧啶碱基交替的DNA区域中。
