Fu X Y, Kessler D S, Veals S A, Levy D E, Darnell J E
Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8555-9. doi: 10.1073/pnas.87.21.8555.
Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.
干扰素刺激基因因子3（ISGF3）是一种依赖配体的转录激活因子，在受到干扰素处理后，它在细胞质中组装，转位至细胞核，并结合共有DNA位点，即干扰素刺激反应元件。我们已经纯化了ISGF3并鉴定了其组成蛋白：一种48 kDa的DNA结合蛋白和三种较大的多肽（84、91和113 kDa），它们本身不具有DNA结合活性。ISGF3的多亚基结构很可能反映了它参与接收依赖配体的信号、转位至细胞核以及结合DNA以激活转录的过程。
