CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, VIC 3168, Australia.
J Phys Chem B. 2012 Mar 22;116(11):3551-6. doi: 10.1021/jp300239g. Epub 2012 Mar 12.
The development of improved methods to allow the low energy production of cubic phase forming nanoparticles (cubosomes) is highly desired. The lamellar to hexagonal and cubic phase change of these lipid nanoparticles has previously been induced via the lowering of pH and the addition of calcium ions to anionic lipid nanoparticles. We have developed a method to produce low polydispersity cubosomes without the requirement of high energy input such as shear, sonication or homogenization under physiological conditions. We have found that the simple addition of phosphate buffered saline solution to aqueous dispersions of cationic liposome vesicles made with phytantriol results in the spontaneous formation of cubosomes after vortex mixing. This finding demonstrates the potential of utilizing this technique to incorporate shear and temperature sensitive compounds into cubosomes under extremely mild conditions for biomedical and nanotechnological applications.
人们非常希望开发出改进的方法来实现低能量生产立方相形成纳米颗粒（cubosomes）。这些脂质纳米颗粒的层状到六方和立方相的转变以前是通过降低 pH 值和向阴离子脂质纳米颗粒添加钙离子来诱导的。我们已经开发出一种在生理条件下无需高能量输入（如剪切、超声或匀浆）即可生产低多分散性 cubosomes 的方法。我们发现，向含有植烷三醇的阳离子脂质体囊泡的水性分散体中简单添加磷酸盐缓冲盐水溶液，在涡旋混合后会自发形成 cubosomes。这一发现表明，该技术有可能在极其温和的条件下将剪切和温度敏感化合物纳入 cubosomes 中，用于生物医学和纳米技术应用。
