Jahanshahi Gholamreza, Sabaghian Mehdi
Department of Oral and Maxillofacial Pathology and Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2012 Jan;9(1):8-12. doi: 10.4103/1735-3327.92920.
Squamous cell carcinoma (SCC) is the most common malignancy in oral cavity. Angiogenesis is essential for the development and progression of SCC. Recently, some studies have reported that mast cells may play a role in tumor progression via promoting angiognesis. Since the results of various studies on the role of mast cells in tumor progression is not uniform, the aim of this study was to define the possible role of mast cells in the process of angiogenesis by determining the microvessel density (MVD) and mast cell density (MCD) and the association between them in oral normal mucsoa and oral SCC.
In this retrospective analytical study, paraffinized specimens from 22 cases of normal mucosa and 20 cases of well-differentiated oral SCC were selected. Microvessels were stained by using immunohistochemical technique with anti-CD34 antibody and mast cells with toluidine blue and then were counted at 400× magnification in hot-spot areas under a light microscope. The results were analyzed by using t- test and Pearson's exams. P values less than 0.05 was considered to be significant.
A significant correlation was noted between MVD and MCD in normal oral mucosa (P<0.001), but in spite of a higher density of mast cells and microvessels observed in oral SCC compared to normal mucosa, there was no significant correlation between them (P=0.731).
These findings showed that factors other than mast cells may play a role in the upregulation of tumor angiogenesis in oral SCC.
鳞状细胞癌（SCC）是口腔中最常见的恶性肿瘤。血管生成对于SCC的发生和发展至关重要。最近，一些研究报道肥大细胞可能通过促进血管生成在肿瘤进展中发挥作用。由于关于肥大细胞在肿瘤进展中作用的各种研究结果并不一致，本研究的目的是通过测定口腔正常黏膜和口腔SCC中的微血管密度（MVD）和肥大细胞密度（MCD）以及它们之间的关联，来确定肥大细胞在血管生成过程中的可能作用。
在这项回顾性分析研究中，选取了22例正常黏膜和20例高分化口腔SCC的石蜡标本。微血管用抗CD34抗体通过免疫组织化学技术染色，肥大细胞用甲苯胺蓝染色，然后在光学显微镜下于400倍放大倍数下在热点区域进行计数。结果采用t检验和Pearson检验进行分析。P值小于0.05被认为具有统计学意义。
在正常口腔黏膜中，MVD和MCD之间存在显著相关性（P<0.001），但尽管与正常黏膜相比，在口腔SCC中观察到肥大细胞和微血管的密度更高，但它们之间没有显著相关性（P=0.731）。
这些发现表明，除肥大细胞外的其他因素可能在口腔SCC肿瘤血管生成的上调中起作用。
