National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
PLoS One. 2012;7(2):e31292. doi: 10.1371/journal.pone.0031292. Epub 2012 Feb 21.
Microarray transcriptome analyses of fetal mouse liver did not detect circadian expression rhythms of clock genes or clock-controlled genes, although some rhythmic transcripts that were likely not driven by endogenous cellular clocks were identified. This finding reveals a key distinction between the circadian oscillators in fetal and adult mouse livers. Thus, in this study, the transcriptomes of fetal and adult livers were systematically compared to identify differences in the gene expression profiles between these two developmental stages. Approximately 1000 transcripts were differentially enriched between the fetal and adult livers. These transcripts represent genes with cellular functions characteristic of distinct developmental stages. Clock genes were also differentially expressed between the fetal and adult livers. Developmental differences in liver gene expression might have contributed to the differences in oscillation status and functional states of the cellular circadian clock between fetal and adult livers.
对胎鼠肝脏的微阵列转录组分析并未检测到时钟基因或时钟控制基因的昼夜节律表达节律，尽管鉴定出了一些可能不受内源性细胞时钟驱动的节律性转录本。这一发现揭示了胎鼠和成年鼠肝脏中昼夜节律振荡器之间的一个关键区别。因此，在这项研究中，系统比较了胎鼠和成年鼠肝脏的转录组，以鉴定这两个发育阶段之间基因表达谱的差异。大约 1000 个转录本在胎鼠和成年鼠肝脏之间差异富集。这些转录本代表了具有不同发育阶段特征的细胞功能的基因。时钟基因在胎鼠和成年鼠肝脏之间的表达也存在差异。肝脏基因表达的发育差异可能导致胎鼠和成年鼠肝脏中细胞生物钟的振荡状态和功能状态存在差异。
