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作为RNA和DNA测序方法的RNA聚合酶合成的特异性终止

Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.

作者信息

Axelrod V D, Vartikyan R M, Aivazashvili V A, Beabealashvili R S

出版信息

Nucleic Acids Res. 1978 Oct;5(10):3549-63. doi: 10.1093/nar/5.10.3549.

DOI:10.1093/nar/5.10.3549
PMID:724495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342694/
Abstract

Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.

摘要

利用大肠杆菌RNA聚合酶全酶和聚[d(A-T)]以及未分级的T7 DδIII DNA作为模板，对3'-O-甲基核苷5'-三磷酸终止RNA合成进行了研究。结果表明，这种终止可用于DNA测序。已确定了从该DNA的AI启动子合成的一部分RNA的序列。还描述了四种3'-O-甲基核苷5'-三磷酸的合成方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/4a026a20d86a/nar00471-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/3ff85fb42217/nar00471-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/dfbb146f7238/nar00471-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/4a026a20d86a/nar00471-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/3ff85fb42217/nar00471-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/dfbb146f7238/nar00471-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0494/342694/4a026a20d86a/nar00471-0114-a.jpg

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本文引用的文献

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