与巨噬细胞共培养可增强子宫内膜异位症基质细胞的克隆形成和侵袭活性。
Co-culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells.
作者信息
Chan Rachel W S, Lee Cheuk-Lun, Ng Ernest H Y, Yeung William S B
机构信息
Department of Obstetrics and Gynaecology, The University of Hong Kong, Hong Kong, China.
Centre of Reproduction, Development of Growth, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
出版信息
Cell Prolif. 2017 Jun;50(3). doi: 10.1111/cpr.12330. Epub 2017 Jan 10.
Abstract
OBJECTIVE
To study the effect on endometrial and endometriotic cells after co-culture with macrophages, using clonogenic, invasion and self-renewal assays.
MATERIALS AND METHODS
Peripheral blood samples, endometrium and endometriotic tissues were collected. Autologous macrophages were co-cultured with endometrial and endometriotic cells. The number of colony-forming units (CFU), invasiveness and self-renewal activity after co-culture with macrophages were determined. The cytokine level of colony-stimulating factor-1 (CSF-1) from macrophages with and without endometriosis was compared.
RESULTS
Co-culture with macrophages significantly increased the clonogenic and invasion ability of endometriotic stromal cells in vitro. Colony-stimulating factor-1 (CSF-1) was up-regulated in endometriotic macrophages conditioned medium when compared to those without the disease.
CONCLUSIONS
These data suggest that macrophages may increase the proliferation and invasion activity of stromal clonogenic cells in women with endometriosis.
摘要
目的
采用克隆形成、侵袭和自我更新分析方法，研究巨噬细胞与子宫内膜细胞和子宫内膜异位症细胞共培养后的影响。
材料与方法
采集外周血样本、子宫内膜和子宫内膜异位症组织。将自体巨噬细胞与子宫内膜细胞和子宫内膜异位症细胞共培养。测定与巨噬细胞共培养后的集落形成单位（CFU）数量、侵袭性和自我更新活性。比较有和没有子宫内膜异位症的巨噬细胞的集落刺激因子-1（CSF-1）细胞因子水平。
结果
与巨噬细胞共培养显著提高了子宫内膜异位症基质细胞在体外的克隆形成和侵袭能力。与无该疾病的巨噬细胞相比，子宫内膜异位症巨噬细胞条件培养基中的集落刺激因子-1（CSF-1）上调。
结论
这些数据表明，巨噬细胞可能会增加子宫内膜异位症女性基质克隆形成细胞的增殖和侵袭活性。
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