高效液相色谱-质谱法测定尿结石中的三聚氰胺
[Determination of melamine by high-performance liquid chromatography-mass spectrometry in urinary calculi].
作者信息
Wu Wenqi, Wang Luping, Ou Lili, Liang Yeping, Mai Zanlin, Li Shujue, Shan Chichang, Yuan Jian, Zeng Guohua
机构信息
Department of Urology, Minimally Invasive Surgery Center, Guangzhou Medical College, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jan;32(1):125-7.
OBJECTIVE
To establish a high-performance liquid chromatography-mass spectrometry (LC/MS)-based method for efficient determination of melamine in urinary calculi in children taking melamine-contaminated formula milk powder.
METHODS
LC/MS was employed to determine the contents of melamine in urinary calculi surgically removed from 17 children with a history of taking melamine-contaminated milk powder and in 4 samples of uric acid stone from adults. The positive ionization mode of electro-spray ionization source was used, and the limit of melamine determination was 0.1 mg/kg.
RESULTS
No melamine was detected in the 4 uric acid stone samples from adults. Melamine was detected in 4 samples of urinary calculi from the 17 children, with the concentration ranging from 0.8 to 64 mg/lkg.
CONCLUSION
LC/MS is simple and effective for detecting melamine in urinary calculi, which is helpful to the treatment and follow-up.
目的
建立一种基于高效液相色谱 - 质谱联用（LC/MS）的方法，用于高效测定食用受三聚氰胺污染配方奶粉的儿童尿结石中的三聚氰胺。
方法
采用LC/MS测定从17名有食用受三聚氰胺污染奶粉病史的儿童手术取出的尿结石以及4例成人尿酸结石样本中三聚氰胺的含量。采用电喷雾电离源的正离子模式，三聚氰胺的测定限为0.1mg/kg。
结果
在4例成人尿酸结石样本中未检测到三聚氰胺。在17名儿童的4例尿结石样本中检测到三聚氰胺，浓度范围为0.8至64mg/kg。
结论
LC/MS检测尿结石中三聚氰胺简单有效，有助于治疗和随访。
Zotero 插件