Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
Center of Excellence in Hepatitis and Liver Cancer, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
Sci Rep. 2023 Mar 2;13(1):3523. doi: 10.1038/s41598-023-30340-5.
A wireless-based detection utilizing an innovative electrochemical card (eCard) sensor controlled by a smartphone was developed for targeting Hepatitis B surface antigen (HBsAg). A simple label-free electrochemical platform allows a convenient operation for point-of-care diagnosis. A disposable screen-printed carbon electrode was modified straightforwardly layer-by-layer with chitosan followed by glutaraldehyde, allowing a simple but effective, reproducible, and stable method for covalently immobilizing antibodies. The modification and immobilization processes were verified by electrochemical impedance spectroscopy and cyclic voltammetry. The smartphone-based eCard sensor was used to quantify HBsAg by measuring the change in current response of the [Fe(CN)] redox couple before and after the presence of HBsAg. Under the optimal conditions, the linear calibration curve for HBsAg was found to be 10-100,000 IU/mL with a detection limit of 9.55 IU/mL. The HBsAg eCard sensor was successfully applied to detect 500 chronic HBV-infected serum samples with satisfactory results, demonstrating the excellent applicability of this system. The sensitivity and specificity of this sensing platform were found to be 97.75% and 93%, respectively. As illustrated, the proposed eCard immunosensor offered a rapid, sensitive, selective, and easy-to-use platform for healthcare providers to rapidly determine the infection status of HBV patients.
一种基于无线的检测方法，利用智能手机控制的创新电化学卡（eCard）传感器，针对乙型肝炎表面抗原（HBsAg）进行了靶向检测。简单的无标记电化学平台允许进行方便的即时护理诊断操作。一次性丝网印刷碳电极通过壳聚糖直接层层修饰，然后用戊二醛修饰，允许采用简单但有效的、可重复的、稳定的方法来共价固定抗体。修饰和固定过程通过电化学阻抗谱和循环伏安法进行了验证。基于智能手机的 eCard 传感器通过测量 [Fe(CN)] 氧化还原对在存在 HBsAg 前后的电流响应变化来定量 HBsAg。在最佳条件下，HBsAg 的线性校准曲线为 10-100,000 IU/mL，检测限为 9.55 IU/mL。该 HBsAg eCard 传感器成功应用于检测 500 份慢性乙型肝炎病毒感染的血清样本，结果令人满意，证明了该系统的优异适用性。该传感平台的灵敏度和特异性分别为 97.75%和 93%。如图所示，所提出的 eCard 免疫传感器为医疗保健提供者提供了一种快速、灵敏、选择性和易于使用的平台，用于快速确定 HBV 患者的感染状态。
