注释和分析在真菌Circinelloides CBS 277.49 中编码多个同工酶的苹果酸酶基因。
Annotation and analysis of malic enzyme genes encoding for multiple isoforms in the fungus Mucor circinelloides CBS 277.49.
机构信息
Center for Systems Biology, Soochow University, Suzhou, 215006, People's Republic of China.
出版信息
Biotechnol Lett. 2012 May;34(5):941-7. doi: 10.1007/s10529-012-0859-x. Epub 2012 Feb 4.
Based on the newly-released genomic data of Mucor circinelloides CBS 277.49, we have annotated five genes encoding for malic enzyme: all code for proteins that contain conserved domains/motifs for malic acid binding, NAD(+) binding and NAD(P)(+) binding. Phylogenetic analysis for malic enzyme genes showed that genes ID 78524 and 11639 share ~80% amino acid identity and are grouped in cluster 1; genes ID 182779, 186772 and 116127 share ~66% amino acid identity are grouped in cluster 2. Genes ID 78524, 11639 and 166127 produce proteins that are localized in the mitochondrion, while the products from genes 182779 and 186772 are localized in the cytosol. Based on the comparative analysis published previously by Song et al. (Microbiology 147:1507-1515, 2001), we propose that malic enzyme genes ID 78524, 166127, 182779, 186772, 11639, respectively, represent protein isoforms I, II, III/IV, V, and VI.
基于新近发布的环扇毛霉 CBS 277.49 的基因组数据，我们注释了编码苹果酸酶的五个基因：所有基因都编码含有保守结构域/基序的蛋白质，用于结合苹果酸、NAD(+)和 NAD(P)(+)。苹果酸酶基因的系统发育分析表明，ID 78524 和 11639 基因具有约 80%的氨基酸同一性，并被归类为第 1 簇；ID 182779、186772 和 116127 基因具有约 66%的氨基酸同一性，被归类为第 2 簇。ID 78524、11639 和 166127 基因产生的蛋白质定位于线粒体，而来自基因 182779 和 186772 的产物定位于细胞质。基于 Song 等人先前发表的比较分析（微生物学 147:1507-1515, 2001），我们提出苹果酸酶基因 ID 78524、166127、182779、186772、11639 分别代表蛋白同工酶 I、II、III/IV、V 和 VI。
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