Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan, China.
Magn Reson Chem. 2012 Mar;50(3):187-92. doi: 10.1002/mrc.2865. Epub 2012 Feb 26.
Chronic unpredictable mild stress (CUMS) is a well-validated model of depression. In this study, a urinary metabonomics method based on the NMR spectrometry was used to study the metabolic perturbation in CUMS-induced rat depression model. With pattern recognition analysis, a clear separation of CUMS rats and healthy controls was achieved, and nine endogenous metabolites contributing to the separation were identified. CUMS-treated rats were characterized by the increase of glycine, pyruvate, glutamine, and asparagines, as well as the decrease of 2-oxoglutarate, dimethylglycine, citrate, succinate, and acetate. The urinary biochemical changes related to the metabolic disturbance in CUMS induced depression, and the possible correlations with live qi stagnation in traditional Chinese medicine are discussed. The work shows that CUMS is a reliable model for studying depression, and the noninvasive urinary metabolomic method is a valuable tool to investigate the biochemical pertubations in depression as an early diagnostic means.
慢性不可预测轻度应激（CUMS）是一种经过充分验证的抑郁症模型。在这项研究中，采用基于 NMR 光谱的尿代谢组学方法研究 CUMS 诱导的大鼠抑郁模型中的代谢紊乱。通过模式识别分析，成功实现了 CUMS 大鼠与健康对照组的明显分离，并鉴定出 9 种参与分离的内源性代谢物。CUMS 处理的大鼠表现为甘氨酸、丙酮酸、谷氨酰胺和天冬酰胺增加，而 2-氧戊二酸、二甲基甘氨酸、柠檬酸、琥珀酸和乙酸减少。与 CUMS 诱导的抑郁相关的尿生化变化与中医所说的气郁有关，并讨论了与气郁的可能相关性。这项工作表明 CUMS 是研究抑郁症的可靠模型，无创性尿代谢组学方法是一种有价值的工具，可作为早期诊断手段，用于研究抑郁症中的生化紊乱。
