Junqueira Juliana C, Vilela Simone F G, Rossoni Rodnei D, Barbosa Júnia O, Costa Anna Carolina B P, Rasteiro Vanessa M C, Suleiman Jamal M A H, Jorge Antonio Olavo C
Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista, São José dos Campos, SP, Brazil.
Rev Inst Med Trop Sao Paulo. 2012 Jan-Feb;54(1):17-24. doi: 10.1590/s0036-46652012000100004.
In HIV-infected patients, colonization of the oral cavity by potential pathogenic yeast may lead to development of systemic fungemia. We evaluated the prevalence of yeast in the oral cavity of Brazilian HIV-positive patients and verified whether or not the species characterized were enzymatically active. Furthermore, the species identified were tested for their susceptibility to antifungal treatment.
Patient saliva and oropharyngeal candidiasis samples were collected from 60 seropositive HIV patients and identified by the API20C system. Enzymatic activity was evaluated by the production of proteinase and phospholipase. Susceptibility to antifungal treatments were determined using the broth microdilution method.
the most commonly isolated species were C. albicans (51.56%) followed by non-albicans Candida species (43.73%), Trichosporon mucoides (3.12%) and Kodamaea ohmeri (1.56%). Oral colonization by association of different species was observed in 42% of the patients. Enzymatic activity was verified in most of species isolated, except for C. glabrata, C. lusitaniae and C. guilliermondii. Resistance to Fluconazole and Amphotericin B was observed in isolates of C. albicans, C. glabrata, C. parapsilosis, C. krusei, and K. ohmeri.
HIV-positive patients are orally colonized by single or multiple species of yeast that are occasionally resistant to Fluconazole or Amphotericin B.
在感染HIV的患者中，潜在致病性酵母在口腔中的定植可能导致全身性真菌血症的发生。我们评估了巴西HIV阳性患者口腔中酵母的患病率，并验证了所鉴定的菌种是否具有酶活性。此外，还对鉴定出的菌种进行了抗真菌治疗敏感性测试。
从60例血清学阳性的HIV患者中采集唾液和口咽念珠菌病样本，并通过API20C系统进行鉴定。通过蛋白酶和磷脂酶的产生来评估酶活性。使用肉汤微量稀释法测定抗真菌治疗的敏感性。
最常分离出的菌种是白色念珠菌（51.56%），其次是非白色念珠菌（43.73%）、粘液丝孢酵母（3.12%）和奥默柯达酵母（1.56%）。42%的患者观察到不同菌种联合引起的口腔定植。除光滑念珠菌、葡萄牙念珠菌和季也蒙念珠菌外，大多数分离出的菌种均检测到酶活性。在白色念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌和奥默柯达酵母的分离株中观察到对氟康唑和两性霉素B的耐药性。
HIV阳性患者口腔中定殖有单一或多种酵母菌种，这些菌种偶尔对氟康唑或两性霉素B耐药。
