Nguyen Dong Tu, Ngo Tuan Cuong, Tran Huy Hoang, Le Thanh Huong, Nguyen Hoai Thu, Nguyen Binh Minh, Tran Nhu Duong, Yamashiro Tetsu, Ehara Masahiko
Department of Bacteriology, National Institute of Hygiene and Epidemiology, No.1 Yersin Street, Hanoi, 10, 000, Vietnam.
Open Microbiol J. 2012;6:14-21. doi: 10.2174/1874285801206010014. Epub 2012 Feb 10.
During the cholera survey in Namdinh province (northern Vietnam) in July, 2010, one strain of Vibrio cholerae O139 was isolated from 7 environmental water samples positive for ctxA, toxR,VCO139 genes and named as V. cholerae O139, ND1 strain. This strain was lysogenic harbouring a genome similar to the filamentous phage fs1. The replicative form DNA of this phage (named as ND1-fs1, 6856 bp) was sequenced and compared with the other filamentous phages. The filamentous phage ND1-fs1 integrates into the region between ctxB and rtxA genes. The genetic organization of the CTXϕ of V. cholerae O139, strain ND1 was determined and the schematic representation of the genetic organization was shown together with the ND1-fs1 prophage.
2010年7月在越南北方南定省进行霍乱调查期间，从7份ctxA、toxR、VCO139基因呈阳性的环境水样中分离出1株霍乱弧菌O139，命名为霍乱弧菌O139 ND1株。该菌株是溶原性的，含有与丝状噬菌体fs1相似的基因组。对该噬菌体的复制型DNA（命名为ND1-fs1，6856 bp）进行测序，并与其他丝状噬菌体进行比较。丝状噬菌体ND1-fs1整合到ctxB和rtxA基因之间的区域。确定了霍乱弧菌O139 ND1株CTXϕ的遗传组织，并展示了遗传组织的示意图以及ND1-fs1前噬菌体。
