Barrera-Mejía Magda, Martínez Simón, Ortega César, Ulloa-Arvizu Raúl
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera de Cuota Toluca-Atlacomulco, Kilómetro 15.5, Código Postal 50200, Toluca, Estado de México.
J Aquat Anim Health. 2011 Dec;23(4):200-6. doi: 10.1080/08997659.2011.644410.
The infectious pancreatic necrosis virus (IPNV; genus Aquabirnavirus) affects salmon and trout, causing high mortality in first-feeding fry. The classification of this virus includes nine serotypes and seven genogroups. In Mexico, two different isolates were identified in 2000 and 2008, respectively. Both isolates were classified into genogroup I according to the RNA genome of this virus. As Mexico is importing rainbow trout Oncorhynchus mykiss eggs from different countries, the aim of this study was to genotype IPNV isolates obtained from four rainbow trout producer regions within the state of Mexico. We utilized a fragment of the VP2* (outer capsid protein) gene sequence of Mexican IPNV isolates as a molecular marker to determine the genogroup to which they belong. Although all Mexican IPNV isolates were grouped into genogroup I, we identified genetic diversity among these isolates, and 14 unique nucleotide sequence types were associated with the four producer regions in Mexico State.
传染性胰腺坏死病毒（IPNV；水生双RNA病毒属）感染鲑鱼和鳟鱼，导致初孵鱼苗高死亡率。该病毒的分类包括9个血清型和7个基因群。在墨西哥，分别于2000年和2008年鉴定出两种不同的分离株。根据该病毒的RNA基因组，这两种分离株均被归类为基因群I。由于墨西哥从不同国家进口虹鳟（Oncorhynchus mykiss）鱼卵，本研究的目的是对从墨西哥州四个虹鳟养殖产区获得的IPNV分离株进行基因分型。我们利用墨西哥IPNV分离株的VP2*（外衣壳蛋白）基因序列片段作为分子标记来确定它们所属的基因群。尽管所有墨西哥IPNV分离株都被归为基因群I，但我们在这些分离株中发现了遗传多样性，并且14种独特的核苷酸序列类型与墨西哥州的四个养殖产区相关。
