Department of Laboratory Diagnostics, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Inflamm Res. 2012 Jun;61(6):591-8. doi: 10.1007/s00011-012-0449-3. Epub 2012 Feb 29.
To explore the potential relationship between previous influenza virus (IV) infection and acute myocardial infarction (AMI), and the mechanism of atherosclerosis, we conducted a case-control study and examined inflammatory cytokines to assess the association of previous IV infection and AMI.
A questionnaire-based survey was conducted to collect information about demographic characteristics and heart disease risk factors. Fasting blood samples were obtained to measure immunoglobulin (Ig) G antibodies to influenza virus A (IV-A), influenza virus B (IV-B), cytomegalovirus, herpes simplex virus type-1 and type-2, adenovirus, rubella virus and Chlamydia pneumoniae, and to measure the level of certain biochemistry markers: interleukin-2, 6, 10 and 18 (IL-2, 6, 10 and 18), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), endothelin-1 (ET-1), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1).
Compared with the controls, the cases were more likely to have positive IgG antibodies to IV-A and IV-B [IV-A: odds ratio (OR): 3.1, 95% confidence interval (CI): 1.5-6.4; IV-B: OR: 10.2, 95% CI: 5.7-20.0]. After adjustment for potential confounding variables, the risk of AMI was still associated with the presence of IgG antibodies to IV-A (adjusted OR: 5.5, 95% CI: 1.3-23.0) and IV-B (adjusted OR: 20.3, 95% CI: 5.6-40.8). The levels of IL-2, 6, 10 and18, TNF-α, IFN-γ, ET-1, sICAM-1 and sVCAM-1 in patients with AMI were significantly higher than those of the controls (P < 0.01).
Our study supports the hypothesis that previous IV infection is associated with AMI. Inflammatory cytokines may take part in the development of atherosclerosis and trigger the occurrence of AMI.
探讨既往流感病毒（IV）感染与急性心肌梗死（AMI）的潜在关系及其与动脉粥样硬化的发生机制。我们进行了一项病例对照研究，并检测了炎症细胞因子，以评估既往 IV 感染与 AMI 的相关性。
采用问卷调查的方式收集人口统计学特征和心脏病危险因素等信息。采集空腹血样，检测流感病毒 A（IV-A）、流感病毒 B（IV-B）、巨细胞病毒、单纯疱疹病毒 1 型和 2 型、腺病毒、风疹病毒和肺炎衣原体的 IgG 抗体，并检测白细胞介素 2、6、10 和 18（IL-2、6、10 和 18）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、内皮素-1（ET-1）、可溶性细胞间黏附分子-1（sICAM-1）和可溶性血管细胞黏附分子-1（sVCAM-1）等生物化学标志物的水平。
与对照组相比，病例组更易出现 IV-A 和 IV-B 的 IgG 抗体阳性[IV-A：比值比（OR）：3.1，95%置信区间（CI）：1.5-6.4；IV-B：OR：10.2，95%CI：5.7-20.0]。调整潜在混杂因素后，AMI 的发病风险仍与 IV-A（调整 OR：5.5，95%CI：1.3-23.0）和 IV-B（调整 OR：20.3，95%CI：5.6-40.8）的 IgG 抗体阳性相关。AMI 患者的 IL-2、6、10 和 18、TNF-α、IFN-γ、ET-1、sICAM-1 和 sVCAM-1 水平明显高于对照组（P<0.01）。
本研究支持既往 IV 感染与 AMI 相关的假说。炎症细胞因子可能参与动脉粥样硬化的发生，并触发 AMI 的发生。
